Anti-Ki67 antibody [SP6] (Ab16667)

推荐稀释比例

• IHC-P1/200 Antigen retrieval: Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). Primary antibody condition: primary antibody incubation overnight at +4°C is recommended.
• mIHC1/200 Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
• ICC/IF1/250 If fixing cells in 4% PFA (10 min, room temp), it is recommended to permeabilized cells with 0.1% Triton-X for 5 min.
• Flow Cyt (Intra)1/1000 ab172730, Rabbit monoclonal isotype, is suitable for use as an isotype control with this antibody.

Anti-Ki67 antibody [SP6] (ab16667) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P, WB and mIHC. Anti-Ki67 antibody [SP6] (ab16667) was first used in a scientific publication in 1977 and has been cited over 2748 times in peer reviewed journals. It's performance in Western blot, immunofluorescence and IHC in human and mouse samples is trusted by the scientific community. Abcam's high quality manufacturing and validation processes ensure Anti-Ki67 antibody [SP6] (ab16667) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. The specificity of Anti-Ki67 antibody [SP6] (ab16667) has been confirmed by Western Blot testing in Ki67 knockout HeLa cells ( ab263762 ). Anti-Ki67 antibody [SP6] (ab16667) has 123 independent reviews from customers. Anti-Ki67 antibody [SP6] (ab16667) specifically detects Ki67 (UniProt ID: P46013; Molecular weight: 359kDa) and is sold in 100 µL, 500 µL and 1 mL selling sizes. Conjugation-ready, carrier free format available for antibody clone SP6 - ab197547. Antibody clone SP6 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor ® 488, Alexa Fluor ® 647, PE, APC ( ab281847, ab281928, ab282173, ab314285 ). Anti-Ki67 antibody [SP6] (ab16667) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC). Ki-67 is a protein that serves as a marker for cell proliferation in oncology. It is widely used to assess the growth rate of cancer cells, helping to determine the aggressiveness of tumors and guide treatment decisions. High levels of Ki-67 are often associated with more aggressive cancers and poorer prognosis Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents. What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies.