Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Ab172730)

应用:WB, IHC, FCM, IF/ICC, IP, ChIP-seq, CUT&RUN-seq

用户验证的应用

推荐稀释比例

  • IHC-P5 µg/mL Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
  • ICC/IFPlease note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used.
  • Flow CytPlease note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used.
  • ChIC/CUT&RUN-seq0.5-2 µg

详细信息

宿主/亚型Rabbit / IgG
抗体类型Monoclonal
全称Rabbit IgG, monoclonal [EPR25A] - Isotype Control
产品形式Liquid
储存缓冲液Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
储存条件运输条件: Conditional Ambient 推荐的短期储存时间: 1-2 weeks 推荐的短期储存条件: +4°C 推荐的长期储存条件: -20°C 储存信息: Avoid freeze / thaw cycle
纯化方式Affinity purification Protein A

背景信息

Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ChIC/CUT&RUN-seq, ChIP-seq, Flow Cyt, ICC/IF, IHC-P, IP, and WB. Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) was first used in a scientific publication in 2014 and has been cited over 617 times in peer reviewed journals. It's performance in Western blot, immunofluorescence, IHC and ChIP in human, mouse and rat samples is trusted by the scientific community. Abcam's high quality manufacturing and validation processes ensure Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) is sold in 200 µg selling sizes. Conjugation-ready, carrier free format available for antibody clone EPR25A - ab210849. Antibody clone EPR25A is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor ® 488, Alexa Fluor ® 647, HRP, Alexa Fluor ® 45, Alexa Fluor ® 594, Alexa Fluor ® 555, PE, Alexa Fluor ® 568, PerCP, FITC, APC, Biotin, Alexa Fluor ® 75 ( ab19991, ab19993, ab19957, ab2815, ab28568, ab28569, ab29478, ab29613, ab22217, ab223339, ab232814, ab3273, ab32286 ). One of the top cited IgG Isotype control antibodies in the market with >1000 citations. IgG Isotype Control antibodies are essential for multiple applications. Isotype Control ab172730 is suitable for ChIC/CUT&RUN-seq, IP, ICC/IF, IHC-P,Flow Cyt, WB. This negative control antibodies help distinguish specific antibody binding from non-specific interactions, ensuring reliable results. Optimize your research with high-quality IgG Isotype Control antibodies for precise data interpretation Immunogen Chemical / Small Molecule conjugated to keyhole limpet haemocyanin. KLH is a copper containing oxygen carrier occurring freely dissolved in the hemolymph of many molluscs and arthropods. KLH forms a large complex composed of ~50 kDa subunits. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents. What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies.

文献引用 (1234)

Developmental convergence and divergence in human stem cell models of autism.

自闭症人类干细胞模型的发育趋同与分化。

Gordon Aaron, Yoon Se-Jin, Bicks Lucy K, Martín Jacqueline M, Pintacuda Greta, Arteaga Stephanie, Wamsley Brie, Guo Qiuyu, Elahi Lubayna, Dolmetsch Ricardo E, Bernstein Jonathan A, O'Hara Ruth, Hallmayer Joachim F, Lage Kasper, Pasca Sergiu P, Geschwind Daniel H

PA28γ-containing tumor-derived exosomes promote T-cell dysfunction in head and neck squamous cell carcinoma.

含有 PA28γ 的肿瘤来源外泌体可促进头颈部鳞状细胞癌中的 T 细胞功能障碍。

Wang Ying, Zhang Shiyu, Yang Yang, Wang Yimei, Zhang Yingxin, Liu Ming, Zeng Xin, Ji Ning, Sun Silu, Li Chunjie, Zhao Yuwei, Zhou Xikun, Chen Qianming, Li Jing

Escherichia coli promotes colorectal cancer metastasis by maintaining enhancer-promoter loops through releasing neutrophil extracellular traps.

大肠杆菌通过释放中性粒细胞胞外陷阱来维持增强子-启动子环,从而促进结直肠癌转移。

Mechanical Strain-Programmed SDC1(+) Sheath Fibroblasts Trigger CXCR4(hi) Neutrophil-Mediated Enthesitis in Ankylosing Spondylitis.

机械应力编程的 SDC1(+) 鞘成纤维细胞触发强直性脊柱炎中 CXCR4(hi) 中性粒细胞介导的附着点炎。

Lin Jiajie, Su Zepeng, Zeng Yipeng, Zhou Yi, Zeng Chenying, Zhang Weihao, Li Qibo, Xiao Zipeng, Chen Zibin, Liu Ziqian, Lin Yangfeng, Zheng Guan, Yu Wenhui, Xie Zhongyu

Notch2 Directs aTreg Cell Fate Toward Immunoregulation or Inflammatory Pyroptosis.

Notch2 决定 Treg 细胞的命运是免疫调节还是炎症性细胞焦亡。

Qiao Yue-Long, Xu Shan, Zou You, Yang Rui, Zheng Han, Liu Jia-Yu, Fan Hui-Ming, Zhang Yuan-Yuan, Kong Yong-Gang, Jiao Wo-Er, Chen Shi-Ming

TNF superfamily member 14 drives post-influenza depletion of alveolar macrophages, enabling secondary pneumococcal pneumonia.

TNF 超家族成员 14 可导致流感后肺泡巨噬细胞耗竭,从而引发继发性肺炎球菌肺炎。

Malainou Christina, Peteranderl Christin, Ferrero Maximiliano Ruben, Vazquez-Armendariz Ana Ivonne, Alexopoulos Ioannis, Franz Katharina, Knippenberg Klara, Better Julian, Estiri Mohammad, Wu Cheng-Yu, Schultheis Hendrik, Bushe Judith, Del Rio Maria-Luisa, Rodriguez-Barbosa Jose Ignacio, Pfeffer Klaus, Günther Stefan, Looso Mario, Gruber Achim Dieter, Vadász István, Matt Ulrich, Herold Susanne

Splicing factor FUS facilitates the progression of PIT1-lineage PitNETs by upregulating MDM2.

剪接因子 FUS 通过上调 MDM2 促进 PIT1 谱系 PitNETs 的进展。

Wang Xu, Li Jiang, Jiang Chenggang, Zhang Chengkai, Yuan Linhao, Zhang Tieqiang, Liu Yuqi, Ma Shunchang, Kang Peng, Li Deling, Guan Xiudong, Chen Jian, Jia Wang

CD8a antibody-functionalized biomimetic red blood cell membrane ectosomes delivering C646 reverse CD8⁺ T Cell exhaustion via H3K18la histone delactylation in gastric cardia adenocarcinoma.

CD8a 抗体功能化的仿生红细胞膜外体递送 C646 逆转 CD8â º T 细胞耗竭,通过 H3K18la 组蛋白去乳酸化治疗胃贲门腺癌。

Cuproptosis promotes inflammatory osteolysis via GYS1-mediated glycogen metabolism.

铜细胞凋亡通过 GYS1 介导的糖原代谢促进炎症性骨溶解。

Zhou Lu, Mao Hanqing, Wen Yuanhao, Chen Zhi, Zhang Lu

Environmental ciprofloxacin triggers pregnancy loss: senescence-driven miscarriage via TRIM21-mediated MFF degradation.

环境中的环丙沙星引发流产:通过 TRIM21 介导的 MFF 降解导致衰老驱动的流产。

Sun 孙义 Yi, Lin 林晏冰 Yanbing, Xing å¹¸å¸ å¸ Shuaishuai, Huang 黄文欣 Wenxin, Wang Yanxin, Guo Geng, Yan Haijun, Zhao Depeng, Cheng Wenli, Wu Zhengzhong, Huang Chenghui, Zhang Zhihong, Wu 吴琳琳 Linlin, Hou 侯青芝 Qingzhi, Zhang å¼ æ §ä¸œ Huidong
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