Anti-F4/80 antibody [BM8] (Ab16911)

推荐稀释比例

• Flow Cyt1/50 (Methanol fixed cells) ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
• IHC-Fr1/50 See Schaller et al. Fixation with acetone for 10 min at RT is recommended as is an incubation with 0.02 M sodium azide in PBS containing 0.1 % H2O2 for 10 min at RT to destroy endogenous peroxidase

Anti-F4/80 antibody [BM8] (ab16911) is a rat monoclonal antibody and is validated for use in Flow Cyt, ICC, IHC in mouse samples. Anti-F4/80 antibody [BM8] (ab16911) has been cited over 152 times in peer reviewed journals and is trusted by the scientific community. Abcam's high quality validation processes ensure Anti-F4/80 antibody [BM8] (ab16911) has high sensitivity and specificity. Anti-F4/80 antibody [BM8] (ab16911) has 12 independent reviews from customers. Anti-F4/80 antibody [BM8] (ab16911) specifically detects F4/80 (UniProt ID: Q14246; Molecular weight: 95kDa) and is sold in 100 µg selling sizes. Antibody clone BM8 is also available pre-conjugated to a variety of labels for your convenience - Biotin, FITC, PE ( ab15694, ab60343, ab125148 ). F4/80 is a well-established marker for murine macrophages, playing a significant role in the immune system. It is involved in various immunological processes, including tissue homeostasis, inflammation, and immune responses. F4/80 is expressed on different macrophage subtypes, which can influence the progression of diseases such as autoimmune disorders, infections, and cancer. Its expression helps in identifying and isolating macrophages for research, aiding in the understanding of their roles in health and disease. It has been recently implicated as a key biomarker in some autoimmune diseases like IBD. ab16911 is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation prcoesses in the pancreas. Furthermore it is a unique histological marker of the progression from peri-insulitis to beta-cell and diabetes in a mouse diabetes model.