Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (Ab176842)

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, HIST1H3C, H3FC, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l

反应种属:Human, Mouse, Rat, Drosophila, Yeast
抗原名称:H3C1
应用:WB, IHC, FCM, IF/ICC, ChIP, Peptide Array

用户验证的应用

推荐稀释比例

  • IHC-P1/5000 - 1/10000 Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • WB1 µg/mL
  • ICC/IF1/2000 - 1/5000
  • ChIP2 µg for 25 µg chromatin

详细信息

免疫原The exact immunogen used to generate this antibody is proprietary information.
宿主/亚型Rabbit / IgG
抗体类型Monoclonal
全称Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade
产品形式Liquid
储存缓冲液Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
储存条件运输条件: Conditional Ambient 推荐的短期储存时间: 1-2 weeks 推荐的短期储存条件: +4°C 推荐的长期储存条件: -20°C 储存信息: Avoid freeze / thaw cycle
纯化方式Affinity purification Protein A

背景信息

What is this antibody validated in? Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ChIP, Peptide Array in Drosophila melanogaster, Human, Mouse, Rat, Saccharomyces cerevisiae, Schizosaccharomyces pombe samples. What is the molecular weight of Histone H3? Anti-Histone H3 [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) specifically detects a band for Histone H3 (UniProt: P68431) at a molecular weight of 15kDa. Trusted by the scientific community Anti-Histone H3 [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) was first used in a scientific publication in 2014 and has been cited over 70 times in peer-reviewed journals. Reviewed by scientists Anti-Histone H3 [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) has over 10 independent reviews from customers. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed The specificity of Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) has been confirmed by Western blot testing in H3C1 Knockout HAP1 cells. Other related products We have a range of other formats of antibody clone [EPR16987] also available for your convenience: ab176842, Alexa Fluor® 647 - ab207543, HRP - ab209023, Carrier free - ab238971, Alexa Fluor® 488 - ab252005, PE - ab314246, Alexa Fluor® 750 - ab321187 Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents. What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies.

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