Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (Ab176877)

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3FC, HIST1H3C, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K4me, H3K4me1, H3K4

反应种属:Human, Mouse, Rat
抗原名称:H3C1 mono methyl K4
应用:WB, IHC, IF/ICC, ChIP, ChIP-seq, CUT&RUN-seq, Peptide Array

用户验证的应用

推荐稀释比例

  • IHC-P1/1000 Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • WB1/5000
  • ICC/IF1/1000
  • ChIPUse 2 μl.
  • ChIC/CUT&RUN-seq2 µg for 10^5 Cells
  • ChIP-seq2 µg for 10^7 Cells

详细信息

免疫原The exact immunogen used to generate this antibody is proprietary information.
宿主/亚型Rabbit / IgG
抗体类型Monoclonal
全称Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade
产品形式Liquid
储存缓冲液Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
储存条件运输条件: Conditional Ambient 推荐的短期储存时间: 1-2 weeks 推荐的短期储存条件: +4°C 推荐的长期储存条件: -20°C 储存信息: Avoid freeze / thaw cycle
纯化方式Affinity purification Protein A

背景信息

What is this antibody validated in? Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (ab176877) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ChIP, Peptide Array in Human, Mouse, Rat samples. What is the molecular weight of Histone H3? Anti-Histone H3 (mono methyl K4) [ERP16597] - ChIP Grade (ab176877) specifically detects a band for Histone H3 (UniProt: P68431) at a molecular weight of 15kDa. Trusted by the scientific community Anti-Histone H3 (mono methyl K4) [ERP16597] - ChIP Grade (ab176877) was first used in a scientific publication in 2014 and has been cited over 20 times in peer-reviewed journals. Reviewed by scientists Anti-Histone H3 (mono methyl K4) [ERP16597] - ChIP Grade (ab176877) has over 5 independent reviews from customers. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Other related products We have a range of other formats of antibody clone [ERP16597] also available for your convenience: ab176877, Carrier free - ab239402, Alexa Fluor® 594 - ab279693, Alexa Fluor® 647 - ab283349 Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents. What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies.

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M(6)A-METTL3 依赖的核 PANC754/PSPC1/H3K4me1 抑制复合物调节免疫逃逸 LGALS7 信号,以增强对结直肠癌的免疫治疗

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