Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 405) (Ab175651)

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

反应种属:Rabbit
抗原名称:Rabbit IgG
应用:IHC, ELISA, FCM, IF/ICC
标记:AF405

用户验证的应用

推荐稀释比例

  • ICC/IF1/200 - 1/1000 We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor ® 405 dye (see difference below) Ex max: Alexa Fluor ® 405 = 402nm / DAPI = 359nm Em max: Alexa Fluor ® 405 = 421nm / DAPI = 461nm
  • Flow Cyt1/2000

详细信息

宿主/亚型Donkey / IgG
抗体类型Polyclonal
全称Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 405)
产品形式Liquid
偶联物Alexa Fluor® 405
储存缓冲液Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
储存条件运输条件: Blue Ice 推荐的短期储存时间: 1-2 weeks 推荐的短期储存条件: +4°C 推荐的长期储存条件: -20°C 储存信息: Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

背景信息

This antibody reacts with the heavy and light chains of Rabbit IgG We recommend storage time at 4°C should be minimal, since this may affect the signal strength. Fluorochrome chart - a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment. When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies, designed to deliver precise and reliable results. Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

文献引用 (74)

Neutrophil-microglia interaction drives motor dysfunction in a neuromyelitis optica model induced by subarachnoid AQP4-IgG.

中性粒细胞-小胶质细胞相互作用导致蛛网膜下腔 AQP4-IgG 诱导的视神经脊髓炎模型出现运动功能障碍。

Qi Fangfang, Lennon Vanda A, Zhao Shunyi, Guo Yong, Ding Husheng, Liu Caiyun, Bartley Whitney M, Chen Tingjun, Lucchinetti Claudia F, Wu Long-Jun

Single-cell omics and flow cytometry identify distinct immune states of dural and brain-infiltrating IL-17-producing γδ T cells after experimental stroke.

单细胞组学和流式细胞术鉴定实验性中风后硬脑膜和脑浸润的 IL-17 产生 α1T 细胞的不同免疫状态。

Zha Mingming, Jander Alina, Cai Haodi, Piepke Marius, Degenhardt Karoline, Winter Leo, Magnus Tim, Gelderblom Mathias

Evolution of a truncated nucleocapsid protein enhances SARS-CoV-2 fitness by suppressing antiviral responses.

截短的核衣壳蛋白的进化通过抑制抗病毒反应来增强 SARS-CoV-2 的适应性。

Mulloy Rory P, Evseev Danyel, Sharlin Noga, Bui-Marinos Maxwell P, Lacasse Émile, Dubuc Isabelle, Flamand Louis, Corcoran Jennifer A

Retinoic acid regulates fetoplacental vascularization via notch signaling and a SEMA3E/F-PLEXIND1 axis.

视黄酸通过 Notch 信号通路和 SEMA3E/F-PLEXIND1 轴调节胎盘血管化。

Cwiek Aleksandra, Paila Umadevi, Markowska Zaneta, Genet Nafiisha, Jackson Madeline, Genet Gael, Cain Shelby R, Aragon Jordon W, Nelson Elizabeth A, Borges Ricardo Moraes, Sutherland Ann E, Hirschi Karen K

Ngn2 and Isl1-mediated astrocyte-to-neuron conversion in vivo promotes functional recovery after spinal cord injury.

体内 Ngn2 和 Isl1 介导的星形胶质细胞向神经元的转化促进脊髓损伤后的功能恢复。

Human Dermal Microvascular Arterial and Venous Blood Endothelial Cells and Their Use in Bioengineered Dermo-Epidermal Skin Substitutes.

人类真皮微血管动脉和静脉血液内皮细胞及其在生物工程真皮-表皮皮肤替代物中的应用

Rütsche Dominic, Nanni Monica, Cheng Phil, Caflisch NicolÃ, Tastanova Aizhan, Jenni Céline, Levesque Mitchell P, Moehrlen Ueli, Klar Agnes S, Biedermann Thomas

BEHAV3D Tumor Profiler to map heterogeneous cancer cell behavior in the tumor microenvironment.

BEHAV3D 肿瘤分析器用于绘制肿瘤微环境中异质性癌细胞行为图。

Rios-Jimenez Emilio, Zomer Anoek, Collot Raphael, Barrera Román Mario, Archidona Sandra F, Ariese Hendrikus, van Ineveld Ravian, Kleinnijenhuis Michiel, Bessler Nils, Johnson Hannah, Dawson Caleb A, Rios Anne, Alieva Maria

Host cellular protein RAB33B facilitates influenza viral replication and modulates M2 trafficking by enhancing autophagy.

宿主细胞蛋白 RAB33B 通过增强自噬促进流感病毒复制并调节 M2 运输

Ye Shaotang, Wang Zhen, Lu Gang, Chen Aolei, Xu Liang, Liu Yongbo, Mao Jianwei, Wang Jingyu, Lou Gaoming, Xie Qingmei, Jia Kun, Li Shoujun

Astrocytes in the External Globus Pallidus Selectively Represent Routine Formation During Repeated Reward-Seeking in Mice.

小鼠在重复寻求奖励的过程中,外侧苍白球中的星形胶质细胞选择性地代表了常规形成

Yang Minsu Abel, Kang Shinwoo, Hong Sa-Ik, Lee Jeyeon, Bormann Nicholas L, Lee Sang Wan, Choi Doo-Sup

Interleukin-1 receptor type 1 inhibition improves blood pressure, fetal growth and immune balance in placental ischemic rats

白细胞介素-1受体1型抑制剂可改善胎盘缺血大鼠的血压、胎儿生长和免疫平衡

Marcus Robbins ,Evangeline Deer ,Deanna G Thompson ,Jie G McKay ,Jan Michael Williams ,Denise C Cornelius
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