Anti-6X His tag® antibody [HIS.H8] (Ab18184)
6 His epitope tag, HHHHHH epitope tag, HHHHHH tag, Hexa His tag, Polyhistidine Tag, 6x his, 6x-HIS
详细信息
特异性
Recognizes His-tagged recombinant proteins or His-tagged proteins overexpressed in cells.ab18184 reacts to recombinant proteins containing the 6X His tag® or 10X His tag® fused to either the amino or carboxy terminus.
背景信息
Anti-6X His tag ® antibody [HIS.H8] (ab18184) is a mouse monoclonal antibody and is validated for use in Dot, ELISA, ICC/IF, IP and WB. Anti-6X His tag ® antibody [HIS.H8] (ab18184) was first used in a scientific publication in 2004 and has been cited over 356 times in peer reviewed journals. It's performance in Western blot in human and mouse samples is trusted by the scientific community. Abcam's high quality validation processes ensure Anti-6X His tag ® antibody [HIS.H8] (ab18184) has high sensitivity and specificity. Anti-6X His tag ® antibody [HIS.H8] (ab18184) has 31 independent reviews from customers. 6X His tag ® antibodies are used to visualize proteins labelled with this tag in a variety of applications (for example imaging and Flow cytometry). To enable specific detection of your tagged protein, Anti-6X His tag ® antibody [HIS.H8] (ab18184) has been validated in Dot, ELISA, ICC/IF, IP and WB. Anti-6X His tag ® antibody [HIS.H8] (ab18184) specifically detects 6X His tag ® (UniProt ID: P26367; Molecular weight: 47kDa) and is sold in 100 ug selling sizes. Antibody clone HIS.H8 is also available pre-conjugated to a variety of labels for your convenience - Biotin ( ab173828 ). Top cited 6X His tag antibody in the market with >450 citations. The 6X His tag, also known as a polyhistidine tag, is widely used for the purification of His-tagged proteins. Utilizing Ni-NTA chromatography, this tag facilitates efficient protein purification, ensuring high yield and purity. It is also highly relevant for detecting 6X His tags in multiple proteomic applications such as western blotting (WB), Immunoprecipitation (IP) and immunocytochemistry/immunofluorescence (ICC/IF) applications. HIS-TAG® is a trademark of EMD Biosciences, Inc.
文献引用 (443)
Dual targeting of AMRC12 and Malassezia globosa disrupts MYC liquid condensates-driven nuclear pore complex biogenesis in neuroblastoma.
双重靶向 AMRC12 和马拉色菌可破坏神经母细胞瘤中 MYC 液态凝聚物驱动的核孔复合体生物合成。
TRIP13 promotes the expansion and immunosuppression of CD4(+)Foxp3(+) regulatory T cells by sustaining HAT1 stability.
TRIP13 通过维持 HAT1 的稳定性来促进 CD4(+)Foxp3(+) 调节性 T 细胞的扩增和免疫抑制。
MYLK4 promotes colorectal cancer progression by regulating lipid metabolism reprogramming via targeting ferroptosis.
MYLK4 通过靶向铁死亡来调节脂质代谢重编程，从而促进结直肠癌的进展。
Intraperitoneal Co-Delivery of Claudin18.2Ã41BB and EpCAMÃCD3 Bispecific Antibodies via mRNA-LNPs Synergistically Suppresses Gastric Cancer Peritoneal Metastasis Through T Cell Co-Activation.
通过 mRNA-LNP 腹腔内共递送 Claudin18.2×41BB 和 EpCAM×CD3 双特异性抗体，可协同抑制胃癌腹膜转移，其机制是通过 T 细胞共激活。
A measles virus encoding a CD19/CD3 bispecific T cell engager shows enhanced preclinical anti-BCP-ALL efficacy without significant toxicity.
编码 CD19/CD3 双特异性 T 细胞衔接器的麻疹病毒显示出增强的临床前抗 BCP-ALL 疗效，且无明显毒性。
E. coli-expressed chicken IL-17A enhances respiratory innate immunity by inducing proinflammatory cytokines and interferons.
大肠杆菌表达的鸡IL-17A通过诱导促炎细胞因子和干扰素来增强呼吸道先天免疫力。
ZmWRKY104-ZmCCaMK interaction enhances brassinosteroid-promoted salt tolerance in maize (Zea mays L.) via antioxidant defense.
ZmWRKY104-ZmCCaMK 相互作用通过抗氧化防御增强玉米（Zea mays L.）中油菜素甾醇促进的耐盐性。
Serpin-Driven Green Camouflage and NIR Fluorescence in Frogs.
丝氨酸蛋白酶抑制剂驱动的青蛙绿色伪装和近红外荧光。
YdbL directly modulates YdbH-YnbE bridge formation to maintain Escherichia coli outer membrane homeostasis.
YdbL 直接调节 YdbH-YnbE 桥的形成，以维持大肠杆菌外膜的稳态。
Fine-tuning of gene expression through the Mettl3-Mettl14-Dnmt1 axis controls ESC differentiation.
通过 Mettl3-Mettl14-Dnmt1 轴对基因表达进行精细调控，从而控制 ESC 分化
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