Anti-Iba1 antibody [EPR16588] - Microglia marker (Ab178846)

推荐稀释比例

• IHC-P1/2000 Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
• WB1/500 - 1/2000 Abcam recommends blocking in 3% milk for cleanest results in WB. Blocking with BSA gives slightly higher background.
• ICC/IF0.1-1 µg/mL
• Flow Cyt (Intra)1/160
• IHC-Fr1/200

Anti-Iba1 antibody [EPR16588] (ab178846) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P and WB. Anti-Iba1 antibody [EPR16588] (ab178846) was first used in a scientific publication in 2016 and has been cited over 439 times in peer reviewed journals. It's performance in IHC, Western Blot and Immunofluorescence in mouse and rat samples is trusted by the scientific community. Abcam's high quality manufacturing and validation processes ensure Anti-Iba1 antibody [EPR16588] (ab178846) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. Anti-Iba1 antibody [EPR16588] (ab178846) has 44 independent reviews from customers. Anti-Iba1 antibody [EPR16588] (ab178846) specifically detects Iba1 (UniProt ID: O70200; Molecular weight: 17kDa) and is sold in 100 µL and 1 mL selling sizes. Conjugation-ready, carrier free format available for antibody clone EPR16588 - ab220815. Antibody clone EPR16588 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor ® 488, Alexa Fluor ® 647, APC, Alexa Fluor ® 750 ( ab225260, ab225261, ab320071, ab321218 ). NEW: Explore our alternative host species for clone [EPR16588]: goat, chicken, rat. Iba1 (AIF1) is a marker for microglia, the brain's primary immune cells and is frequently used in Alzheimer's disease (AD) research. In AD brains, altered Iba1 expression indicates microglial activation and neuroinflammation. Researchers use Iba1 to assess microglial response and motility in AD models, making it essential for understanding the role of inflammation and immune response in Alzheimer's pathology. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents. What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies.