Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (Ab176916)

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, H3FC, HIST1H3C, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K9me3, H3K9me, H3K9

反应种属:Human, Mouse, Rat
抗原名称:H3C1 tri methyl K9
应用:WB, IHC, IF/ICC, ChIP, CUT&Tag, ChIP-seq, CUT&RUN-seq, Dot, Peptide Array

用户验证的应用

推荐稀释比例

  • IHC-P1/500
  • WB1/1000
  • ICC/IF1/2000
  • ChIP2 µg for 25 µg chromatin Use SAT-alpha ChIP primer pair ab269263 as positive control.
  • ChIC/CUT&RUN-seq2 µg
  • ChIP-seq4 µg for 10^7 Cells
  • CUT&Tag1/100
  • Dot1/1000

详细信息

免疫原The exact immunogen used to generate this antibody is proprietary information.
宿主/亚型Rabbit / IgG
抗体类型Monoclonal
全称Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade
产品形式Liquid
储存缓冲液Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
储存条件运输条件: Conditional Ambient 推荐的短期储存时间: 1-2 weeks 推荐的短期储存条件: +4°C 推荐的长期储存条件: -20°C 储存信息: Avoid freeze / thaw cycle
纯化方式Affinity purification Protein A

背景信息

Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in CUT&Tag, ChIC/CUT&RUN-seq, ChIP, ChIP-seq, Dot, ICC/IF, IHC-P, PepArr, WB in human, mouse, rat samples. Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916) has been cited over 54 times in peer reviewed journals and is trusted by the scientific community. Abcams high quality manufacturing and validation processes ensure Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916) has 9 independent reviews from customers. Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916) specifically detects Histone H3 Tri Methyl-K9 (UniProt ID: P68431; Molecular weight: 15kDa) and is sold in 100 uL and 1 mL selling sizes. Conjugation-ready, carrier free format available for antibody clone EPR16601 - ab232324. Antibody clone EPR16601 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647, Alexa Fluor® 555, Alexa Fluor® 594, PE, APC, Alexa Fluor® 75 ( ab284635, ab288339, ab32226, ab32227, ab32228, ab32229, ab32119 ). Histone H3 (tri methyl K9), also known as H3K9me3, is an important epigenetic modification associated with heterochromatin formation and gene silencing. This antibody is crucial in cancer research, particularly in understanding H3K9me3's role in gene silencing and heterochromatin formation, and is widely used in studies of transcriptional repression and histone modifications involving histone methyltransferases. Key histone marker that has been implicated in both transcriptional activation and silencing. The tri methyl K9 antibody is essential for studying this modification's role in transcription regulation, DNA repair, and chromosomal stability. Abnormal H3K9me3 levels are linked to various diseases, including cancer and neurological disorders. Monitoring H3K9me3 can provide valuable insights into disease mechanisms and potential therapeutic targets. Validated for CUT&Tag, CUT&RUN and ChIP-seq, which are key applications to map protein-DNA interactions on a genome-wide scale using NGS. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents. What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies.

文献引用 (111)

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SLIT2 as a key regulator and therapeutic target in liver injury.

SLIT2 是肝损伤的关键调节因子和治疗靶点。

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异莲心碱抑制线粒体自噬,使 T 细胞恶性肿瘤对 STING 介导的 NK 清除更加敏感。

Broad Epigenetic Shifts in the Aging Drosophila Retina Contribute to Its Altered Diurnal Rhythmic Transcriptome.

衰老果蝇视网膜中广泛的表观遗传变化导致其昼夜节律转录组发生改变。

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Isocitrate dehydrogenase 1 primes group-3 medulloblastomas for cuproptosis

异柠檬酸脱氢酶 1 启动 3 组髓母细胞瘤发生铜凋亡

Derek Dang ,Akash Deogharkar ,John McKolay ,Kyle S Smith ,Pooja Panwalkar ,Simon Hoffman ,Wentao Tian ,Sunjong Ji ,Ana P Azambuja ,Siva Kumar Natarajan ,Joanna Lum ,Jill Bayliss ,Katie Manzeck ,Stefan R Sweha ,Erin Hamanishi ,Matthew Pun ,Diya Patel ,Sagar Rau ,Olamide Animasahun ,Abhinav Achreja ,Martin P Ogrodzinski ,Jutta Diessl ,Jennifer Cotter ,Debra Hawes ,Fusheng Yang ,Robert Doherty ,Andrea T Franson ,Allison R Hanaford ,Charles G Eberhart ,Eric H Raabe ,Brent A Orr ,Robert J Wechsler-Reya ,Brandon Chen ,Costas A Lyssiotis ,Yatrik M Shah ,Sophia Y Lunt ,Ruma Banerjee ,Alexander R Judkins ,John R Prensner ,Carl Koschmann ,Sebastian M Waszak ,Deepak Nagrath ,Marcos Simoes-Costa ,Paul A Northcott ,Sriram Venneti

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Loss of Kmt2c or Kmt2d primes urothelium for tumorigenesis and redistributes KMT2A-menin to bivalent promoters

Kmt2c 或 Kmt2d 的缺失会使尿路上皮细胞更容易发生肿瘤,并将 KMT2A-menin 重新分布到二价启动子。

Naitao Wang # ,Mohini R Pachai # ,Dan Li # ,Cindy J Lee ,Sarah Warda ,Makhzuna N Khudoynazarova ,Woo Hyun Cho ,Guojia Xie ,Sagar R Shah ,Li Yao ,Cheng Qian ,Elissa W P Wong ,Juan Yan ,Fanny V Tomas ,Wenhuo Hu ,Fengshen Kuo ,Sizhi P Gao ,Jiaqian Luo ,Alison E Smith ,Ming Han ,Dong Gao ,Kai Ge ,Haiyuan Yu ,Sarat Chandarlapaty ,Gopakumar V Iyer ,Jonathan E Rosenberg ,David B Solit ,Hikmat A Al-Ahmadie ,Ping Chi ,Yu Chen

DNA methylation influences human centromere positioning and function.

DNA甲基化影响人类着丝粒的定位和功能。

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Delivering LINE1 antisense oligonucleotides via endothelial targeting extracellular vesicles to ameliorate myocardial infarction-induced cardiac senescence.

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Mammalian H4K16ac regulates the spatiotemporal order of genome replication rather than gene expression

哺乳动物H4K16ac调控基因组复制的时空顺序,而非基因表达。

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