Anti-Histone H3 (acetyl K27) antibody [EP16602] - ChIP Grade (Ab177178)

推荐稀释比例

• IHC-P1/1500 - 1/10000 Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
• WB1/10000 - 1/100000
• ICC/IF1/7000
• Flow Cyt (Intra)1/1500
• ChIP2 µg for 25 µg chromatin Use GAPDH ChIP primer pair ab267832 as positive control.
• ChIP-seq4 µg/cells for 7 µg/cells
• ChIC/CUT&RUN-seq5 µg

Anti-Histone H3 (acetyl K27) antibody [EP16602] - ChIP Grade (ab177178) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ChIC/CUT&RUN-seq, ChIP, ChIP-seq, Flow Cyt (Intra), ICC/IF, IHC-P, PepArr, WB in human, mouse, rat samples. Anti-Histone H3 (acetyl K27) antibody [EP16602] - ChIP Grade (ab177178) has been cited over 78 times in peer reviewed journals and is trusted by the scientific community. Abcams high quality manufacturing and validation processes ensure Anti-Histone H3 (acetyl K27) antibody [EP16602] - ChIP Grade (ab177178) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. Anti-Histone H3 (acetyl K27) antibody [EP16602] - ChIP Grade (ab177178) has 8 independent reviews from customers. Anti-Histone H3 (acetyl K27) antibody [EP16602] - ChIP Grade (ab177178) specifically detects Histone H3 Acetyl-K27 (UniProt ID: P68431; Molecular weight: 15kDa) and is sold in 100 uL and 1 mL selling sizes. Conjugation-ready, carrier free format available for antibody clone EP16602 - ab32877. Antibody clone EP16602 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647, Alexa Fluor® 555, Alexa Fluor® 594, Alexa Fluor® 75, APC, PE ( ab245911, ab245912, ab321196, ab321197, ab321198, ab321199, ab3212 ). H3K27ac is a key epigenetic mark associated with gene activation and chromatin remodeling. The use of anti h3k27ac antibody and anti histone antibody can significantly enhance the detection and study of this modification. Research indicates that H3K27ac plays a crucial role in various cancers, making it a valuable target for understanding cancer progression and developing new therapeutic strategies. This antibody is also validated for ChIP-seq and CUT&RUN-seq, which are key applications to map protein-DNA interactions on a genome-wide scale using NGS. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents. What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies.