Anti-NeuN antibody [EPR12763] - Neuronal Marker (Ab177487)

推荐稀释比例

• IHC-P1/3000 For unpurified use at 1/800. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
• mIHC1/1000 Perform Sodium citrate antigen retrieval (pH 6.0) in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
• WB1/1000 - 1/10000 For unpurified use at 1/1000 - 1/2000.
• ICC/IF1/100 - 1/300 For unpurified use at 1/80.
• Flow Cyt (Intra)1/100 ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
• IHC-FrPerform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC (PFA fixed), IHC-Fr, IHC-P, WB, mIHC in cat, common marmoset, dog, human, mouse, rat, sheep, zebrafish samples. Anti-NeuN antibody (anti-RBFOX3 antibody) [EPR12763] - Neuronal Marker (ab177487) has been cited over 868 times in peer reviewed journals and is trusted by the scientific community. Abcam's high quality manufacturing and validation processes ensure NeuN antigen antibody (ab177487) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487) has 72 independent reviews from customers. Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487) specifically detects NeuN (UniProt ID: A6NFN3; Molecular weight: 34kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL). Conjugation-ready, carrier free format available for antibody clone EPR12763 - ab209898. Antibody clone EPR12763 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647, Biotin, Alexa Fluor® 594 ( ab190195, ab190565, ab204681, ab207279 ). NeuN (RBFOX3) is a protein extensively used as a neuronal marker due to its expression in the nuclei and cytoplasm of neurons. It is vital for the regulation of alternative splicing of pre-mRNA, which is essential for neural development and function. Mutations or dysregulation of RBFOX3 have been associated with various neurological conditions, including autism spectrum disorder, cognitive deficits, and epilepsy, highlighting its importance in maintaining normal brain function. Browse our curated portfolio of extensively validated antibodies and assays, with antibodies to over 90% of key targets in major glioblastoma pathways– including EGF, VEGF, MAPK, Wnt, NFKB, and PI3K/AkT/MTOR – all in one place. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents. What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies.