用户验证的应用
经验证应用
| 项目 | 详情 |
|---|---|
| Positive WB detected in | HeLa cells, HepG2 cells, L02 cells, HUVEC cells, HGC-27 cells, NIH/3T3 cells, HSC-T6 cells |
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse colon tissue |
| Positive IF/ICC detected in | A549 cells |
详细信息
免疫原CatNo: Ag24942
Product name: Recombinant human Vinculin protein
Source: e coli.-derived, PGEX-4T
Tag: GST
Domain: 777-1066 aa of BC039174
Sequence: PKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ
宿主/亚型Rabbit / IgG
抗体类型Recombinant
产品类型Antibody
产品形式Liquid
偶联物Unconjugated
纯化方式Protein A purification
储存缓冲液PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage.
RRIDAB_3745213
UniProt IDP18206
Gene ID7414
背景信息
Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein that is involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein that is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is a muscle-specific protein.