Anti-beta III Tubulin antibody - Neuronal Marker (Ab18207)

特异性

The immunogen used for this product shares 75% homology with TUB (Tubby protein homolog, Uniprot: P50607). In western blot, we observe a specific band at ~55kDa which is not seen in KO cell lines. An additional band below this band of interest is seen at ~50kDa in both the WT and KO cells which could correspond to the protein TUB. Please note that cross-reactivity with this protein has not been confirmed experimentally. TUB is localized notably in high concentrations in the nucleoli of brain neurons with lower protein levels in the cytoplasm. Please, therefore, be aware that ICC experiments may need to be optimised. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. As an alternative antibody, we would recommend our recombinant rabbit monoclonal antibody ab52623 which has been shown to specific in both WB and ICC using KO cells.

Anti-beta III Tubulin antibody - Neuronal Marker (ab18207) is a rabbit polyclonal antibody and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P, WB. Anti-beta III Tubulin antibody - Neuronal Marker (ab18207) has been cited over 627 times in peer reviewed journals and is trusted by the scientific community. Abcam's high quality validation processes ensure Anti-beta III Tubulin antibody - Neuronal Marker (ab18207) has high sensitivity and specificity. The specificity of Anti-beta III Tubulin antibody - Neuronal Marker (ab18207) has been confirmed by testing in knockout samples. Anti-beta III Tubulin antibody - Neuronal Marker (ab18207) has 67 independent reviews from customers. beta III Tubulin antibodies are often used as loading controls in Western Blot. Anti-beta III Tubulin antibody - Neuronal Marker has been verified in Western Blot samples and detects a band at 50kDa Molecular weight. Anti-beta III Tubulin antibody - Neuronal Marker (ab18207) specifically detects beta III Tubulin (UniProt ID: Q13509; Molecular weight: 50kDa) and is sold in 100 µg selling sizes. Beta-tubulin III (TUBB3) is a protein that is predominantly expressed in neurons and is a critical component of the microtubule network. It plays a vital role in maintaining the structure and function of neurons. It is a key marker for neurons and is involved in processes such as axon guidance and neuronal differentiation. Abnormal expression of TUBB3 has been associated with several neurological conditions, including congenital fibrosis of the extraocular muscles (CFEOM) and cortical dysplasia, complex with other brain malformations (CDCBM), underscoring its critical role in brain development. Browse our curated portfolio of extensively validated antibodies and assays, with antibodies to over 90% of key targets in major glioblastoma pathways– including EGF, VEGF, MAPK, Wnt, NFKB, and PI3K/AkT/MTOR – all in one place.