Anti-Oct4 antibody [EPR17929] - ChIP Grade (Ab181557)

OCT3, OCT4, OTF3, POU5F1, Octamer-binding protein 3, Octamer-binding protein 4, Octamer-binding transcription factor 3, Oct-3, Oct-4, OTF-3

反应种属:Human, Mouse
抗原名称:POU5F1
应用:WB, IHC, FCM, IF/ICC, IP, ChIP, ChIP-seq

详细信息

免疫原The exact immunogen used to generate this antibody is proprietary information.
宿主/亚型Rabbit / IgG
抗体类型Monoclonal
产品形式Liquid
纯化方式Affinity purification Protein A
储存缓冲液Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
储存条件运输条件: Conditional Ambient 推荐的短期储存时间: 1-2 weeks 推荐的短期储存条件: +4°C 推荐的长期储存条件: -20°C 储存信息: Avoid freeze / thaw cycle
无载体蛋白No

特异性

Oct4 is highly expressed in undifferentiated embryonic stem cells and cancer stem cell-like cells (PMID: 26013162, 21826175). This antibody can't detect the target band in undifferentiated cancer cell lines with low expression level of Oct4, such as HeLa, HEK-293, MDA-MB-231, HepG2, Huh7, HCT-116 and PANC-1 (PMID: 21975933, 29789579, 25625591, 26059097, 23928699, 27344963, 25837691, 29254202, 28854261, 27996162), even at the dilution of 1:200.

背景信息

Product Specifications Anti-Oct4 antibody [EPR17929] - ChIP Grade (ab181557) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ChIP, ChIP-seq, Flow Cyt (Intra), ICC/IF, IHC-P, IP, WB in human, mouse samples. Anti-Oct4 antibody [EPR17929] - ChIP Grade (ab181557) specifically detects Oct4 (UniProt ID: Q01860; Molecular weight: 39kDa) and is sold in 100 µL and 1 mL selling sizes. Quality and Validation Abcam's high quality manufacturing and validation processes ensure Anti-Oct4 antibody [EPR17929] - ChIP Grade (ab181557) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. Anti-Oct4 antibody [EPR17929] - ChIP Grade (ab181557) has been cited over 138 times in peer reviewed journals and is trusted by the scientific community. Anti-Oct4 antibody [EPR17929] - ChIP Grade (ab181557) has 19 independent reviews from customers. Related Products Antibody clone EPR17929 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 594, Alexa Fluor® 555, PE, Alexa Fluor® 488, Alexa Fluor® 647 ( ab210458, ab210657, ab283731, ab283741, ab300092 ). Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents. What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies.

文献引用 (190)

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