用户验证的应用
经验证应用
| 项目 | 详情 |
|---|---|
| Positive WB detected in | Raji cells, Daudi cells, Ramos cells |
| Positive IHC detected in | human tonsil tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
| Positive IF/ICC detected in | Raji cells |
详细信息
免疫原CatNo: Eg3335
Product name: Recombinant Human CD19 protein (rFc Tag)
Source: mammalian cells-derived, pHZ-KIsec-C-rFc
Tag: C-rFc
Domain: 20-291 aa of BC006338
Sequence: PEEPLVVKVEEGDNAVLQCLKGTSDGPTQQLTWSRESPLKPFLKLSLGLPGLGIHMRPLAIWLFIFNVSQQMGGFYLCQPGPPSEKAWQPGWTVNVEGSGELFRWNVSDLGGLGCGLKNRSSEGPSSPSGKLMSPKLYVWAKDRPEIWEGEPPCLPPRDSLNQSLSQDLTMAPGSTLWLSCGVPPDSVSRGPLSWTHVHPKGPKSLLSLELKDDRPARDMWVMETGLLLPRATAQDAGKYYCHRGNLTMSFHLEITARPVLWHWLLRTGGWK
宿主/亚型Rabbit / IgG
抗体类型Recombinant
产品类型Antibody
产品形式Liquid
偶联物Unconjugated
纯化方式Protein A purification
储存缓冲液PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage.
RRIDAB_3744634
UniProt IDP15391
Gene ID930 ENSEMBL Gene ID ENSG00000177455
背景信息
CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).