用户验证的应用
经验证应用
| 项目 | 详情 |
|---|---|
| Positive WB detected in | HGC-27 cells, HUVEC cells, LNCaP cells, MKN-45 cells, K-562 cells, human peripheral blood platelets, rat heart tissue, mouse heart tissue, rabbit heart tissue, HeLa cells |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | hTERT-RPE1 cells |
推荐稀释说明
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. Sample-dependent, Check data in validation data gallery.
详细信息
免疫原CatNo: Ag24946
Product name: Recombinant human Vinculin protein
Source: e coli.-derived, PET30a
Tag: 6*His
Domain: 777-1066 aa of BC039174
Sequence: PKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ
宿主/亚型Mouse / IgG1
抗体类型Monoclonal
产品类型Antibody
产品形式Liquid
偶联物Unconjugated
纯化方式Protein G purification
储存缓冲液PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage.
RRIDAB_3670375
UniProt IDP18206
Gene ID7414
背景信息
Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein that is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.