CD44 抗体 (YA801) (HY-P80602)

CD44; LHR; MDU2; MDU3; MIC4; CD44 antigen; CDw44; Epican; Extracellular matrix receptor III; ECMR-III; GP90 lymphocyte homing/adhesion receptor; HUTCH-I; Heparan sulfate proteoglycan; Hermes antigen; Hyaluronate receptor; Phagocytic glycopr

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反应种属：Human

抗原名称：CD44

应用：WB, IF/ICC, IP, IHC-F, IHC-P

标记：Unconjugated

用户验证的应用详细信息背景信息

用户验证的应用

经验证应用

项目	详情
WB	Western blot analysis of extracts from RAW264.7(lane 2(20ug),HUVEC(lane 3(20ug) and J774A.1(lane 4(20ug) using CD44 Antibody ( HY-P80602 ) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
ICC	Immunocytochemistry analysis of HeLa cells labeling CD44 with CD44 antibody ( HY-P80602 ) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD44 antibody ( HY-P80602 ) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). Immunocytochemistry analysis of HeLa cells labeling CD44 with CD44 antibody ( HY-P80602 ) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD44 antibody ( HY-P81070 ) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

项目

详情

Western blot analysis of extracts from RAW264.7(lane 2(20ug),HUVEC(lane 3(20ug) and J774A.1(lane 4(20ug) using CD44 Antibody ( HY-P80602 ) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

ICC

Immunocytochemistry analysis of HeLa cells labeling CD44 with CD44 antibody ( HY-P80602 ) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD44 antibody ( HY-P80602 ) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). Immunocytochemistry analysis of HeLa cells labeling CD44 with CD44 antibody ( HY-P80602 ) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD44 antibody ( HY-P81070 ) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

详细信息

免疫原Synthetic peptide corresponding to Human CD44.The exact sequence is proprietary to MCE.

宿主/亚型Mouse / IgG

抗体类型Monoclonal

产品形式溶液

偶联物Non-conjugated

纯化方式affinity purified

浓度不同批次浓度可能有所不同，具体浓度请参见对应批次的COA。批次浓度查询

储存缓冲液Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

储存条件保存条件 & 期限: Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles. 运输条件: Shipping with blue ice.

RRIDAB_3102623

UniProt IDP16070

Gene ID960 [ NCBI ]

背景信息

功能： CD44 是一种透明质酸受体和细胞黏附分子，广泛参与细胞迁移、免疫细胞活化、干细胞维持以及肿瘤发生发展。CD44 既是记忆 T 细胞的重要标志物，也是间充质干细胞和癌症干细胞研究中的经典表面标志物。亚细胞定位：细胞膜；单次跨膜 I 型膜蛋白；细胞突起，微绒毛；分泌型表达水平：组织特异性：在成纤维细胞和尿液中检测到 (蛋白水平) (PubMed:25326458, PubMed:36213313, PubMed:37453717) 。在胎盘中检测到 (蛋白水平) (PubMed:32337544) 。异构体 10 (上皮异构体) 由上皮细胞表达，并在癌细胞中高表达。在神经母细胞瘤细胞中表达受到抑制。异构体 & 翻译后修饰： CD44 有 19 种异构体：P16070-1：81538 Da (预测值)；P16070-2：3327 Da (预测值)；P16070-3：77983 Da (预测值)；P16070-4：76612 Da (预测值)；P16070-5：80790 Da (预测值)；P16070-6：76705 Da (预测值)；P16070-7：78446 Da (预测值)；P16070-8：74388 Da (预测值)；P16070-9：74196 Da (预测值)；P16070-10：53411 Da (预测值)；P16070-11：46565 Da (预测值)。 P16070-12：39416 Da (预测值)；P16070-13：46261 Da (预测值)；P16070-14：43169 Da (预测值)；P16070-15：32075 Da (预测值)；P16070-16：73150 Da (预测值)；P16070-17：75957 Da (预测值)；P16070-18：37278 Da (预测值)；P16070-19：15635 Da (预测值)。在多种细胞系和肿瘤中，该蛋白在细胞外基质中被特异性蛋白酶 (可能是 MMPs) 水解；N-糖基化；O-糖基化；含有硫酸软骨素聚糖，其硫酸化程度可能有所不同，其数量可能影响特定蛋白酶对其切割位点的可及性。目前尚不确定 O-糖基化发生在 Thr-637 还是 Thr-638；磷酸化；PKC 激活导致 Ser-706 (组成型磷酸化位点) 去磷酸化，以及 Ser-672 磷酸化。亚基：与 PKN2 相互作用。通过其 N 端片段与透明质酸 (HA) 以及其他糖胺聚糖、胶原蛋白、层粘连蛋白和纤连蛋白相互作用。与 UNC119 相互作用。与 PDPN 相互作用 (通过胞外结构域)；这种相互作用是 PDPN 介导的定向迁移以及细胞铺展和迁移过程中伪足延伸/稳定调控所必需的 (PubMed:20962267)。与 EGFR 相互作用。