CD163 抗体 (YA5791) (HY-P86099)

CD163, M130, Scavenger receptor cysteine-rich type 1 protein M130, Hemoglobin scavenger receptor

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反应种属：Human, Mouse, Rat

抗原名称：CD163

应用：WB, ELISA, IF/ICC, IP, IHC-P

标记：Unconjugated

用户验证的应用详细信息背景信息

用户验证的应用

经验证应用

项目	详情
WB	Western blot analysis of extracts from RAW264.7 (lane2(20μg), 3T3(lane3(20μg), HL-60(lane4(20μg) and C6(lane5(20μg) using CD163 Antibody (YA5791)( HY-P86099 ). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
IHC-P	Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using CD163 Antibody (HY-P86099, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using CD163 Antibody (HY-P86099, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. Immunohistochemical analysis of paraffin-embedded human lymph node tissue using CD163 Antibody (HY-P86099, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. Immunohistochemical analysis of paraffin-embedded human spleen tissue using CD163 Antibody (HY-P86099, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. Immunohistochemical analysis of paraffin-embedded human skin tissue using CD163 Antibody (HY-P86099, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. Immunohistochemical analysis of paraffin-embedded human lung tissue using CD163 Antibody (HY-P86099, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

项目

详情

Western blot analysis of extracts from RAW264.7 (lane2(20μg), 3T3(lane3(20μg), HL-60(lane4(20μg) and C6(lane5(20μg) using CD163 Antibody (YA5791)( HY-P86099 ). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

IHC-P

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using CD163 Antibody (HY-P86099, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using CD163 Antibody (HY-P86099, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. Immunohistochemical analysis of paraffin-embedded human lymph node tissue using CD163 Antibody (HY-P86099, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. Immunohistochemical analysis of paraffin-embedded human spleen tissue using CD163 Antibody (HY-P86099, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. Immunohistochemical analysis of paraffin-embedded human skin tissue using CD163 Antibody (HY-P86099, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. Immunohistochemical analysis of paraffin-embedded human lung tissue using CD163 Antibody (HY-P86099, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

详细信息

宿主/亚型Rabbit / IgG

抗体类型Monoclonal

产品形式溶液

偶联物Non-conjugated

纯化方式Protein A

浓度不同批次浓度可能有所不同，具体浓度请参见对应批次的COA。批次浓度查询

储存缓冲液Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

储存条件保存条件 & 期限: Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles. 运输条件: Shipping with blue ice.

RRIDAB_3719077

UniProt IDQ86VB7

Gene ID9332 [ NCBI ]

背景信息

功能： CD163 是一种主要表达于单核细胞和巨噬细胞表面的血红蛋白清道夫受体，通过介导 Hb-Hp 复合物清除和诱导抗炎通路发挥作用。在免疫研究中，CD163 是 M2 样巨噬细胞、肿瘤相关巨噬细胞 (TAM) 以及组织修复型巨噬细胞的重要标志物。亚细胞定位：分泌型；细胞膜；单次跨膜 I 型膜蛋白表达水平：组织特异性：该蛋白在单核细胞和成熟巨噬细胞中表达，例如肝脏中的库普弗细胞、脾脏中的红髓巨噬细胞、胸腺中的皮质巨噬细胞、骨髓驻留巨噬细胞以及中枢神经系统的脑膜巨噬细胞。血液中也有表达。同工型 1 在血液中含量最低。同工型 2 在肝脏和脾脏中含量最低。同工型 3 是血液中检测到的主要同工型。诱导：受抗炎介质 (如糖皮质激素、白细胞介素-6/IL-6 和白细胞介素-10/IL-10) 诱导；受促炎介质 (如细菌脂多糖 (LPS) 、IFN-γ/IFN-γ 和 TNF) 抑制异构体 & 翻译后修饰： CD163 有 4 种异构体：Q86VB7-1：125451 Da (预测值)；Q86VB7-2：125982 Da (预测值)；Q86VB7-3：121609 Da (预测值)；Q86VB7-4：124958 Da (预测值)。可溶性形式 (sCD163) 由蛋白水解脱落产生，脂多糖、佛波酯和免疫球蛋白 γ 的 Fc 区均可诱导该过程。这种切割依赖于蛋白激酶 C 和酪氨酸激酶，并可被蛋白酶抑制剂阻断。金属蛋白酶组织抑制剂 TIMP3 可抑制该脱落过程，因此该过程可能由膜结合金属蛋白酶 ADAMs 诱导；磷酸化亚基：与 CSNK2B 相互作用