Acetyl Coenzyme A Carboxylase 抗体 (YA644)(PBS only) (HY-P80528A)

ACC; ACAC; ACC2; ACCA; ACACAD

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反应种属：Human

抗原名称：Acetyl Coenzyme A Carboxylase

应用：WB, IF/ICC, IHC-F, IHC-P

标记：Unconjugated

用户验证的应用详细信息背景信息

用户验证的应用

经验证应用

项目	详情
WB	Western blot analysis was performed on extracts from HepG2 (lane 1, 15 μg) using Acetyl Coenzyme A Carboxylase Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
IHC-P	Immunohistochemical analysis of paraffin-embedded human Colon cancer tissue using Acetyl Coenzyme A Carboxylase antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80528, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium. Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using Acetyl Coenzyme A Carboxylase antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80528, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium. Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using Acetyl Coenzyme A Carboxylase antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80528, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium. Immunohistochemical analysis of paraffin-embedded human Cervical cancer‌ tissue using Acetyl Coenzyme A Carboxylase antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80528, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium. Immunohistochemical analysis of paraffin-embedded human Liver cancer‌ tissue using Acetyl Coenzyme A Carboxylase antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80528, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium. Immunohistochemical analysis of paraffin-embedded human Tonsil‌ tissue using Acetyl Coenzyme A Carboxylase antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80528, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

项目

详情

Western blot analysis was performed on extracts from HepG2 (lane 1, 15 μg) using Acetyl Coenzyme A Carboxylase Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

IHC-P

Immunohistochemical analysis of paraffin-embedded human Colon cancer tissue using Acetyl Coenzyme A Carboxylase antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80528, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium. Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using Acetyl Coenzyme A Carboxylase antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80528, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium. Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using Acetyl Coenzyme A Carboxylase antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80528, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium. Immunohistochemical analysis of paraffin-embedded human Cervical cancer‌ tissue using Acetyl Coenzyme A Carboxylase antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80528, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium. Immunohistochemical analysis of paraffin-embedded human Liver cancer‌ tissue using Acetyl Coenzyme A Carboxylase antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80528, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium. Immunohistochemical analysis of paraffin-embedded human Tonsil‌ tissue using Acetyl Coenzyme A Carboxylase antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80528, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

详细信息

免疫原Synthetic peptide corresponding to Human Acetyl Coenzyme A Carboxylase.The exact sequence is proprietary to MCE.

宿主/亚型Rabbit / IgG

全称Acetyl Coenzyme A Carboxylase 抗体 (YA644)(PBS only)

理论分子量277 kDa

实际/观测分子量277 kDa 请注意：因蛋白存在修饰或聚体等情况，以实测为准，预测仅为参考。

产品形式溶液

偶联物Non-conjugated

储存缓冲液Supplied in PBS, pH 7.4.

储存条件保存条件 & 期限: Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles. 运输条件: Shipping with blue ice.

纯化方式affinity purified

Gene ID32 [ NCBI ]

UniProt IDO00763

背景信息

功能：乙酰辅酶 A 羧化酶是一种线粒体酶，催化乙酰辅酶 A 羧化生成丙二酰辅酶 A，在脂肪酸代谢中发挥核心作用。它催化的反应分为两步：首先是 ATP 依赖的生物素羧化反应，生物素由生物素羧基载体 (BCC) 结构域携带，随后羧基从羧化的生物素转移到乙酰辅酶 A 上。它生成的丙二酰辅酶 A 会别构抑制线粒体上的肉碱棕榈酰转移酶 1，从而负调控脂肪酸氧化。它与参与脂肪酸从头生物合成的胞质同工酶 ACACA 共同作用，促进脂质储存 [1] [2] [3] [4] [5] [6] 。亚细胞定位：线粒体表达水平：组织特异性：该基因广泛表达于心脏、骨骼肌、肝脏、脂肪组织、乳腺、肾上腺和结肠中，其中表达水平最高 (PubMed:9099716) 。异构体 3 在骨骼肌、脂肪组织和肝脏中表达 (蛋白质水平) (PubMed:19190759) 。异构体 3 在脂肪组织中含量较高，在心脏、肝脏、骨骼肌和睾丸中的含量较低 (PubMed:19190759) 。异构体 & 翻译后修饰： O00763 有 3 种异构体：O00763-1：276541 Da (预测值)；O00763-2：268166 Da (预测值)；O00763-3：255093 Da (预测值)。生物素辅因子以共价键连接到中央生物素结合域，是催化活性所必需的；AMPK 对 Ser-222 位点的磷酸化会使该酶失活 (PubMed：12488245)。维持骨骼肌脂质和葡萄糖稳态是必需的 (基于相似性)。亚基：单体、同源二聚体和同源四聚体 (PubMed:18772397, PubMed:20952656)。形成丝状聚合物 (PubMed:19900410, PubMed:20457939, PubMed:20952656)。与 MID1IP1 相互作用；与 MID1IP1 的相互作用促进寡聚化，并以柠檬酸依赖的方式增强其活性 (PubMed:20457939, PubMed:20952656)。