TATA Box Binding Protein 抗体 (YA3260)(PBS only) (HY-P83515A)

TBP; GTF2D1; TF2D; TFIID; TATA-box-binding protein; TATA sequence-binding protein; TATA-binding factor; TATA-box factor; Transcription initiation factor TFIID TBP subunit

文献引用 (0) 收藏复制产品信息

反应种属：Human, Mouse

抗原名称：TBP

应用：WB, IF/ICC, IP, IHC-F, IHC-P

标记：Unconjugated

用户验证的应用详细信息背景信息

用户验证的应用

经验证应用

项目	详情
WB	Western blot analysis of extracts from HepG2(lane 2(20ug), Hela(lane 3(20ug) and HEK293(lane 4(20ug) using TATA Box Binding Protein Antibody ( HY-P83515 ) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
IHC-P	Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue using TATA Box Binding Protein Antibody (YA3260). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83515, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

项目

详情

Western blot analysis of extracts from HepG2(lane 2(20ug), Hela(lane 3(20ug) and HEK293(lane 4(20ug) using TATA Box Binding Protein Antibody ( HY-P83515 ) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

IHC-P

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue using TATA Box Binding Protein Antibody (YA3260). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83515, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

详细信息

免疫原A synthetic peptide of human TATA binding protein TBP aa411-426.

宿主/亚型Rabbit / IgG

全称TATA Box Binding Protein 抗体 (YA3260)(PBS only)

理论分子量38 kDa

实际/观测分子量38 kDa 请注意：因蛋白存在修饰或聚体等情况，以实测为准，预测仅为参考。

产品形式液体

偶联物Non-conjugated

储存缓冲液Supplied in PBS, pH 7.4.

储存条件保存条件 & 期限: Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles. 运输条件: Shipping with blue ice.

纯化方式Affinity Purified

Gene ID6908 [ NCBI ]

UniProt IDP20226

背景信息

功能： TATA 盒结合蛋白：基础转录因子 TFIID 复合物在 RNA 聚合酶 II (Pol II) 依赖的转录起始过程中发挥核心作用。TFIID 通过其亚基 TBP (TATA 盒结合蛋白) 识别并结合含或不含 TATA 盒的启动子，促进前起始复合物 (PIC) 的组装。TFIID 复合物由 TBP 和 TBP 相关因子 (TAF) 组成，包括 TAF1、TAF2、TAF3、TAF4、TAF5、TAF6、TAF7、TAF8、TAF9、TAF10、TAF11、TAF12 和 TAF13。TFIID 的复合物结构可分为 TFIID-A、TFIID-B 和 TFIID-C 三个模块。TBP 与 TAF3、TAF5 共同组成 TFIID-A 模块。TBP 是位于 TFIID 复合物核心的通用转录因子。在启动子上组装核心前起始复合物的过程中，TBP 作为 TFIID 的组分之一结合启动子 DNA 并使其发生弯曲，且该过程不依赖启动子是否含有 TATA 盒。是含 BRF2 的转录因子复合物的组分，该复合物调控 RNA 聚合酶 III 介导的转录。是转录因子 SL1/TIF-IB 复合物的组分，后者参与 RNA 聚合酶 I 依赖的转录过程中前起始复合物的组装。前起始复合物的形成速率可能主要取决于 SL1 与核糖体 DNA (rDNA) 启动子的结合速率。SL1 可稳定核仁转录因子 1 (UBTF) 在 rDNA 上的结合 [1] [2] [3] [4] [5] [6] [7] [8] [9] 。亚细胞定位：核表达水平：组织特异性：广泛表达，在睾丸和卵巢中含量最高。异构体 & 翻译后修饰： P20226 有 2 个异构体：P20226-1：37698 Da (预测)；P20226-2：35657 Da (预测)。亚基：以单体形式结合 DNA (PubMed:2194289, PubMed:2374612)。TFIID 基础转录因子复合物的组成部分，该复合物由 TATA 盒结合蛋白 TBP 和多个 TBP 相关因子 (TAF) 组成，包括 TAF1、TAF2、TAF3、TAF4、TAF5、TAF6、TAF7、TAF8、TAF9、TAF10、TAF11、TAF12 和 TAF13 (PubMed:27007846, PubMed:33795473, PubMed:9836642)。含有 TFIID 的 RNA 聚合酶 II 起始前复合物的一部分，由 TBP 和至少 GTF2A1、GTF2A2、GTF2E1、GTF2E2、GTF2F1、GTF2H2、GTF2H3、GTF2H4、GTF2H5、GTF2B、TCEA1、ERCC2、ERCC3、TAF1、TAF2、TAF3、TAF4、TAF5、TAF6、TAF7、TAF8、TAF9、TAF10、TAF11、TAF12 和 TAF13 组成 (PubMed:27007846、PubMed:27193682、PubMed:33795473)。转录因子 SL1/TIF-IB 复合物的组成部分，由 TBP 和至少 TAF1A、TAF1B、TAF1C 和 TAF1D 组成 (PubMed:7801123)。TBP 与 TFIID 或 SL1/TIF-IB 的结合似乎是互斥的 (PubMed:7801123)。与 TAF1A、TAF1B 和 TAF1C 相互作用 (PubMed:7801123)。与 TFIIB、NCOA6、DRAP1、DR1 和 ELF3 相互作用 (PubMed:10391676, PubMed:10567404, PubMed:11461703)。与 SPIB、SNAPC1、SNAPC2 和 SNAPC4 相互作用 (PubMed:10196196, PubMed:12621023)。与 UTF1 相互作用 (PubMed:9748258)。与 BRF2 相互作用；这种相互作用促进 BRF2 募集到含有 TATA 盒的启动子 (PubMed:11564744, PubMed:26638071)。与 UBTFD 相互作用 (PubMed:7982918)。与 GPBP1D 相互作用 (基于相似性)。与 CITED2 相互作用 (基于相似性)。与 ATF7IP 相互作用 (可能)。与 LLPH 相互作用 (基于相似性)。与 HSF1 相互作用 (通过转录激活结构域)(PubMed:11005381)。与 GTF2B 相互作用 (通过 C 端)；这种与启动子结合的 TBP 的相互作用引导 RNA 聚合酶 II 进入起始前复合物 (PIC)(PubMed:8504927)。与 PAX5 相互作用 (PubMed：10197586)。与 MSX1 相互作用；该相互作用可能抑制 MSX1 的自身失活 (基于相似性)。