S100A9 抗体(YA3464) (HY-P83734)

Leukocyte L1 complex heavy chain antibody; 60B8AG antibody; CAGB antibody; Calgranulin B antibody; Calgranulin-B antibody; Calprotectin L1H subunit antibody; CFAG antibody; CGLB antibody; Cystic fibrosis antigen B antibody; L1AG antibody; Leukocyte L1 complex heavy chain antibody; LIAG antibody; MAC387 antibody; MIF antibody; Migration inhibitory factor related protein 14 antibody; Migration inhibitory factor-related protein 14 antibody; MRP 14 antibody; MRP-14 antibody; MRP14 antibody; Myeloid-

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反应种属：Human

抗原名称：S100A9

应用：IF/ICC, MIHC, IHC-P

标记：Unconjugated

用户验证的应用详细信息背景信息

用户验证的应用

经验证应用

项目	详情
IHC-P	Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using S100A9 Antibody (HY-P83734, 1/5000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using S100A9 Antibody (HY-P83734, 1/5000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using S100A9 Antibody (HY-P83734, 1/5000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using S100A9 Antibody (HY-P83734, 1/5000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. Immunohistochemical analysis of paraffin-embedded human skin cancer tissue using S100A9 Antibody (HY-P83734, 1/5000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using S100A9 Antibody (HY-P83734, 1/5000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

项目

详情

IHC-P

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using S100A9 Antibody (HY-P83734, 1/5000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using S100A9 Antibody (HY-P83734, 1/5000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using S100A9 Antibody (HY-P83734, 1/5000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using S100A9 Antibody (HY-P83734, 1/5000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. Immunohistochemical analysis of paraffin-embedded human skin cancer tissue using S100A9 Antibody (HY-P83734, 1/5000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using S100A9 Antibody (HY-P83734, 1/5000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

详细信息

免疫原Synthetic peptide within Human S100A9 aa 1-42 / 114.

宿主/亚型Rabbit / IgG

抗体类型Monoclonal,Recombinant

产品形式溶液

偶联物Non-conjugated

纯化方式Affinity Purified

浓度不同批次浓度可能有所不同，具体浓度请参见对应批次的COA。批次浓度查询

储存缓冲液Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

储存条件保存条件 & 期限: Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles. 运输条件: Shipping with blue ice.

RRIDAB_3676645

UniProt IDP06702

Gene ID6280 [ NCBI ]

背景信息

功能： S100A9 是一种钙结合 S100 家族蛋白，主要由中性粒细胞和单核细胞释放，它通常与 S100A8 形成 S100A8/A9 钙卫蛋白异二聚体，参与先天免疫炎症反应 [1] 。机制上，胞外 S100A9 与 TLR4 结合，CD14 作为辅助受体，诱导单核细胞中 NF-κB 激活和促炎细胞因子反应 [2] 。S100A8/A9 复合物还通过 TLR4、RAGE、NF-κB 和 MAPK 通路激活炎症信号通路，从而将白细胞募集、细胞因子分泌、感染防御和炎症相关疾病模型联系起来 [1] [3] 。在肿瘤模型中，S100A9-TLR4 信号通路促进肿瘤生长，而 S100A9 缺陷、TLR4 缺陷或 ABR-215050/tasquinimod 联合治疗则抑制 EL4 淋巴瘤的生长 [4] 。与相关亚型相比，S100A9 与 S100A8 和 S100A12 的不同之处在于，其最显著的炎症作用通常依赖于与 S100A8 的异二聚化以及受体特异性 TLR4/CD14 信号通路 [1] [2] [3] 。在实验应用中，paquinimod 可降低胶原酶诱导的高滑膜激活型骨关节炎中的滑膜激活、骨赘形成和软骨损伤 [5] 。亚细胞定位：分泌的；细胞质；细胞质、细胞骨架；细胞膜；外周膜蛋白表达水平：组织特异性：钙卫蛋白 (S100A8/9) 主要在髓系细胞中表达。除炎症状态外，其表达仅限于髓系分化的特定阶段，因为这两种蛋白均在循环中性粒细胞和单核细胞中表达，但在正常组织巨噬细胞和淋巴细胞中则不存在。在慢性炎症状态下，例如银屑病和恶性肿瘤，表皮中也会表达钙卫蛋白。在炎症局部部位或患有炎症性疾病 (例如类风湿性关节炎、囊性纤维化、炎症性肠病、克罗恩病、巨细胞动脉炎、干燥综合征、系统性红斑狼疮和进行性系统性硬化症) 的血清中，钙卫蛋白的浓度较高。钙卫蛋白参与衰老前列腺中淀粉样蛋白 (称为淀粉样小体包涵体) 的形成和沉积。在多种肿瘤中均有显著上调，包括胃癌、食管癌、结肠癌、胰腺癌、膀胱癌、卵巢癌、甲状腺癌、乳腺癌和皮肤癌。阳性样本：Human cervical cancer, human breast cancer tissue, human spleen tissue. 亚基：同源二聚体 (PubMed:11851337, PubMed:16258195)。优先以异源二聚体或异源四聚体的形式与 S100A8 结合，称为钙卫蛋白 (S100A8/A9)(PubMed:16258195, PubMed:17553524, PubMed:19087201, PubMed:19122197, PubMed:25417112, PubMed:8423249, PubMed:9083090)。