CD47 抗体 (YA531) (HY-P80065)

CD47, MER6, Leukocyte surface antigen CD47, Antigenic surface determinant protein OA3, Integrin-associated protein, Protein MER6, IAP

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反应种属：Human

抗原名称：CD47

应用：IHC-P

标记：Unconjugated

用户验证的应用详细信息背景信息

用户验证的应用

经验证应用

项目	详情
IHC-P	Immunohistochemical analysis of paraffin-embedded human cervix tissue using CD47 Antibody (HY-P80065, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. Immunohistochemical analysis of paraffin-embedded human endometrium tissue using CD47 Antibody (HY-P80065, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. Immunohistochemical analysis of paraffin-embedded human small intestine tissue using CD47 Antibody (HY-P80065, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using CD47 Antibody (HY-P80065, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. Immunohistochemical analysis of paraffin-embedded human ovarian cancer using CD47 Antibody (HY-P80065, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. Immunohistochemical analysis of paraffin-embedded human prostate cancer using CD47 Antibody (HY-P80065, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

项目

详情

IHC-P

Immunohistochemical analysis of paraffin-embedded human cervix tissue using CD47 Antibody (HY-P80065, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. Immunohistochemical analysis of paraffin-embedded human endometrium tissue using CD47 Antibody (HY-P80065, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. Immunohistochemical analysis of paraffin-embedded human small intestine tissue using CD47 Antibody (HY-P80065, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using CD47 Antibody (HY-P80065, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. Immunohistochemical analysis of paraffin-embedded human ovarian cancer using CD47 Antibody (HY-P80065, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. Immunohistochemical analysis of paraffin-embedded human prostate cancer using CD47 Antibody (HY-P80065, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

详细信息

免疫原Synthetic peptide corresponding to Human CD47.AA range:50-150.

宿主/亚型Rabbit / IgG

抗体类型Recombinant,Monoclonal

产品形式溶液

偶联物Non-conjugated

纯化方式Protein A affinity purified.

浓度不同批次浓度可能有所不同，具体浓度请参见对应批次的COA。批次浓度查询

储存缓冲液Supplied in 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide

储存条件保存条件 & 期限: Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles. 运输条件: Shipping with blue ice.

RRIDAB_3102814

UniProt IDQ08722

Gene ID961 [ NCBI ]

背景信息

功能： CD47 是一种糖蛋白，于 1990 年首次被鉴定为与整合素相关的蛋白质，因此其原名为整合素相关蛋白。CD47 与 SIRPα (CD172a 或 SHPS-1) 及其家族成员 SIRPγ 相互作用，CD47 还与整合素和分泌性糖蛋白细胞外基质蛋白血小板反应蛋白-1 (TSP-1) 相互作用。通过 CD47/SIRPα 轴的信号传导在各种稳态过程中发挥作用，例如维持红细胞、先天免疫细胞和 T 细胞。此外，癌细胞和病毒感染和细菌感染的细胞可以上调 CD47 蛋白表达，以防止免疫介导的消除。亚细胞定位：细胞膜；多通道膜蛋白表达水平：组织特异性：广泛分布于正常成人组织以及卵巢肿瘤中，尤其在某些上皮组织和脑组织中含量丰富。巨噬细胞 (PubMed：39121194) 诱导：损伤或应激后，肺动脉和肺实质中可诱导该基因表达 (PubMed:27742621) 。动脉中的表达在正常衰老过程中增加 (PubMed:32679764) 。异构体 & 翻译后修饰： Q08722 有 4 种异构体：Q08722-1：35214 Da (预测)； Q08722-2：31743 Da (预测)； Q08722-3：33192 Da (预测)； Q08722-4：33845 Da (预测)。亚基：单体 (PubMed:18657508)。与 THBS1 相互作用 (通过 C 端结构域)(PubMed:19004835，PubMed:8550562)。与 SIRPA 相互作用 (PubMed:11509594)。与 FAS/CD95 相互作用；功能激活可能增强这种相互作用 (PubMed:15917238)。与 SIRPG、UBQLN1 和 UBQLN2 相互作用 (PubMed:10549293，PubMed:15383453，PubMed:18657508)。可能与纤维蛋白原相互作用 (PubMed:22079249)。与埃及伊蚊中性粒细胞刺激因子 1 相互作用；该相互作用导致巨噬细胞吞噬活性受到抑制 (PubMed:39121194)