用户验证的应用
经验证应用
| 项目 | 详情 |
|---|---|
| WB | Western blot analysis of extracts from U251 (lane2(20μg), Rat brain tissue (lane3(20μg), Mouse brain tissue (lane4(20μg) and HEK293 (lane5(20μg) using GFAP Antibody ( HY-P80140 ). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature. |
| IHC-P | Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue using GFAP Antibody (YA416). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80140, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
| ICC | Immunocytochemistry analysis of U251 cells labeling GFAP with GFAP Antibody ( HY-P80140 )at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with GFAP Antibody ( HY-P80140 ) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). Immunocytochemistry analysis of U251 cells labeling GFAP with GFAP Antibody ( HY-P80140 ) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with GFAP Antibody ( HY-P80140 ) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). |
详细信息
免疫原Synthetic peptide corresponding to Human GFAP.AA range:1-50.
宿主/亚型Rabbit / IgG
抗体类型Recombinant,Monoclonal
产品形式液体
偶联物Non-conjugated
纯化方式Protein A affinity purified.
浓度不同批次浓度可能有所不同，具体浓度请参见对应批次的COA。 批次浓度查询
储存缓冲液Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.
储存条件保存条件 & 期限: Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
运输条件: Shipping with blue ice.
RRIDAB_3102091
UniProt IDP14136
Gene ID2670 [ NCBI ]
背景信息
功能： 胶质纤维酸性蛋白 (GFAP) 是一种 III 型中间丝，是细胞特异性标志物，在中枢神经系统发育过程中，可将星形胶质细胞与其他胶质细胞区分开。 亚细胞定位： 细胞质 表达水平： 组织特异性：在缺乏纤连蛋白的细胞中表达 亚基： 与 SYNM 相互作用