用户验证的应用
经验证应用
| 项目 | 详情 |
|---|---|
| WB | Western blot analysis of extracts from Mouse liver tissue (lane2(20μg), Mouse spleen tissue (lane3(20μg), HepG2 (lane4(20μg) and HEK293 (lane5(20μg) using Albumin Antibody ( HY-P80010 ). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature. |
| IHC-P | Immunohistochemical analysis of paraffin-embedded rat liver tissue using Albumin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
详细信息
免疫原Synthetic peptide corresponding to Human Albumin.AA range:150-190.
宿主/亚型Rabbit / IgG
抗体类型Recombinant,Monoclonal
产品形式溶液
偶联物Non-conjugated
纯化方式Protein A affinity purified.
浓度不同批次浓度可能有所不同，具体浓度请参见对应批次的COA。 批次浓度查询
储存缓冲液Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.
储存条件保存条件 & 期限: Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
运输条件: Shipping with blue ice.
RRIDAB_3102799
UniProt IDP02768
Gene ID213 [ NCBI ]
背景信息
功能： 白蛋白可结合水、钙离子、钠离子、钾离子、脂肪酸、激素、胆红素和药物 (可能性较高)。其主要功能是调节血液的胶体渗透压 (可能性较高)。它是血浆中主要的锌转运蛋白，通常结合约 80%的血浆锌。它也是血浆中主要的钙和镁转运蛋白，结合血浆中约 45%的循环钙和镁。它可能拥有两个以上的钙结合位点，还可能以非特异性方式结合钙。锌和钙在第 273 位天冬氨酸残基处共享结合位点，这表明血液中锌和钙的转运之间存在交互作用。结合亲和力的排序为锌>钙>镁。它可结合细菌铁载体肠杆菌素，抑制大肠杆菌通过肠杆菌素介导的从铁传递蛋白摄取铁的过程，从而可能限制大肠杆菌等肠道细菌对铁的利用和生长。它不会阻止细菌铁载体气杆菌素对铁的摄取 [1] [2] 。 亚细胞定位： 分泌物 表达水平： 组织特异性：等离子体 亚基： 与 FCGRT 相互作用；这种相互作用调节 ALB 的稳态 (PubMed:28330995)。与 TASOR 相互作用 (基于相似性)。在血浆中，以共价键与生色团结合的 α-1-微球蛋白形成复合物，摩尔比分别为 1:2 和 1:1；这种相互作用并不阻止脂肪酸与 ALB 的结合。