Acetyl-Histone H3 (Lys4) 抗体 (YA641)(PBS only) (HY-P80531A)

H3K4ac; H3/j; H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FJ; H3C10; H3C11; HIST1H3J

文献引用 (0) 收藏复制产品信息

反应种属：Human, Mouse

抗原名称：Acetyl-Histone H3 (Lys4)

应用：WB, IF/ICC

标记：Unconjugated

用户验证的应用详细信息背景信息

用户验证的应用

经验证应用

项目	详情
WB	Western blot analysis of extracts from Hela(lane 2(20μg) and Hela (lane 3(20μg)), Trichostatin A treated (HY-15144, 1μM, 18hrs) as indicated, using Acetyl-Histone H3 (Lys4) ( HY-P80531 ) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80531, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

项目

详情

Western blot analysis of extracts from Hela(lane 2(20μg) and Hela (lane 3(20μg)), Trichostatin A treated (HY-15144, 1μM, 18hrs) as indicated, using Acetyl-Histone H3 (Lys4) ( HY-P80531 ) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80531, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

详细信息

免疫原Synthetic peptide corresponding to residues surrounding Lys4 of Human Human Histone H3.The exact sequence is proprietary to MCE.

宿主/亚型Rabbit / IgG

全称Acetyl-Histone H3 (Lys4) 抗体 (YA641)(PBS only)

理论分子量15 kDa

实际/观测分子量15 kDa 请注意：因蛋白存在修饰或聚体等情况，以实测为准，预测仅为参考。

产品形式溶液

偶联物Non-conjugated

储存缓冲液Supplied in PBS, pH 7.4.

储存条件保存条件 & 期限: Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles. 运输条件: Shipping with blue ice.

纯化方式affinity purified

Gene ID8350 [ NCBI ]

UniProt IDP68431

背景信息

功能：乙酰化组蛋白 H3 是核小体的核心组分。核小体将 DNA 缠绕并压缩为染色质，限制了以 DNA 为模板的细胞机器对 DNA 的可及性。因此，组蛋白在转录调控、DNA 修复、DNA 复制和染色体稳定性中发挥核心作用。DNA 的可及性通过一系列复杂的组蛋白翻译后修饰 (也称为组蛋白密码) 以及核小体重塑来调控。亚细胞定位：细胞核；染色体亚基：核小体是由组蛋白八聚体组成，包含两个 H2A、两个 H2B、两个 H3 和两个 H4 分子，组装成一个 H3-H4 异源四聚体和两个 H2A-H2B 异源二聚体。该八聚体包裹约 147 bp 的 DNA。核小体与 TONSL、CHAF1A、CHAF1B、MCM2 和 DNAJC9 相互作用 (PubMed:33857403)。核小体还与 NASP 相互作用；NASP 是一种组蛋白伴侣，能够稳定并维持可溶性组蛋白 H3-H4 二聚体 (PubMed:22195965)。