LAMP2 抗体 (YA310)(PBS only) (HY-P80740A)

LAMP2; Lysosome-associated membrane glycoprotein 2; LAMP-2; Lysosome-associated membrane protein 2; CD107 antigen-like family member B; CD107b

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反应种属：Human

抗原名称：LAMP2

应用：WB, IHC-P

标记：Unconjugated

用户验证的应用详细信息背景信息

用户验证的应用

经验证应用

项目	详情
WB	Western blot analysis of extracts from Hela(lane 2(20μg), Jurkat (lane 3(20μg),HepG2(lane 4(20μg)and HUVEC( lane 5(20μg) using LAMP2 Antibody ( HY-P80740 ). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.
IHC-P	Immunohistochemical analysis of paraffin-embedded human Endometrial Carcinoma tissue using LAMP2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80740, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium. Immunohistochemical analysis of paraffin-embedded human cholangiocarcinoma tissue using LAMP2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80740, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium. Immunohistochemical analysis of paraffin-embedded human Ovarian Cancer‌ tissue using LAMP2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80740, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium. Immunohistochemical analysis of paraffin-embedded human Prostate Cancer tissue using LAMP2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80740, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

项目

详情

Western blot analysis of extracts from Hela(lane 2(20μg), Jurkat (lane 3(20μg),HepG2(lane 4(20μg)and HUVEC( lane 5(20μg) using LAMP2 Antibody ( HY-P80740 ). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.

IHC-P

Immunohistochemical analysis of paraffin-embedded human Endometrial Carcinoma tissue using LAMP2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80740, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium. Immunohistochemical analysis of paraffin-embedded human cholangiocarcinoma tissue using LAMP2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80740, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium. Immunohistochemical analysis of paraffin-embedded human Ovarian Cancer‌ tissue using LAMP2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80740, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium. Immunohistochemical analysis of paraffin-embedded human Prostate Cancer tissue using LAMP2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80740, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

详细信息

免疫原Synthetic peptide corresponding to Human LAMP2.The exact sequence is proprietary to MCE.

宿主/亚型Rabbit / IgG

全称LAMP2 抗体 (YA310)(PBS only)

理论分子量45 kDa

实际/观测分子量110 kDa 请注意：因蛋白存在修饰或聚体等情况，以实测为准，预测仅为参考。

产品形式溶液

偶联物Non-conjugated

储存缓冲液Supplied in PBS, pH 7.4.

储存条件保存条件 & 期限: Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles. 运输条件: Shipping with blue ice.

纯化方式affinity purified

Gene ID3920 [ NCBI ]

UniProt IDP13473

背景信息

功能： LAMP2 是一种溶酶体膜糖蛋白，在溶酶体生物发生、溶酶体 pH 调控和自噬中发挥重要作用。它可直接抑制质子通道 TMEM175，是溶酶体管腔 pH 调控的重要调节因子，促进溶酶体酸化以保证水解酶达到最佳活性。它在分子伴侣介导的自噬中发挥重要作用，该过程可响应各种应激介导溶酶体对蛋白质的降解，同时也是生物学半衰期较长的蛋白质正常更新的一部分。其功能机制是结合靶蛋白 (如 GAPDH、NLRP3 和 MLLT11)，并将这些蛋白靶向运送到溶酶体进行降解。在分子伴侣介导的自噬中，它作用于 HSPA8/HSC70 等分子伴侣的下游：这些分子伴侣识别并结合底物蛋白，介导底物蛋白被招募到溶酶体，随后靶蛋白与 LAMP2 结合。它在响应饥饿的溶酶体蛋白质降解过程中发挥作用。是自噬过程中自噬体与溶酶体融合所必需的。缺失 LAMP2 的细胞中 VAMP8 表达水平正常，但无法在自噬体上积累 STX17，这是自噬体与溶酶体无法融合的最可能原因。是正常降解自噬体内容物所必需的。通过其在溶酶体蛋白质降解中的功能，保障 MHC II 类介导的外源性抗原高效呈递：内体/溶酶体区室中的蛋白酶产生的抗原肽会被新合成的 MHC II 亚基捕获。它不参与 MHC II 类介导的内源性抗原的高效呈递；可调节分子伴侣介导的自噬，减少 MHC II 对内源性抗原的呈递。不参与 MHC II 对外源性抗原和膜来源抗原的呈递；(微生物感染相关) 它可同时与弗林蛋白酶 (FURIN) 以及腮腺炎病毒融合蛋白 F 的未加工形式结合 (不结合加工后的形式)，从而支持 FURIN 介导的腮腺炎病毒融合蛋白 F 的切割 [1] [2] [3] [4] [5] [6] [7] [8] [9] 。亚细胞定位：溶酶体膜；I 型单次跨膜蛋白；内体膜；I 型单次跨膜蛋白；细胞膜；I 型单次跨膜蛋白；细胞质囊泡，自噬体膜表达水平：组织特异性：LAMP-2A 亚型在胎盘、肺和肝脏中高表达，在肾脏和胰腺中表达较低，在脑和骨骼肌中表达极低 (PubMed:26856698, PubMed:7488019) 。LAMP-2B 亚型在脾脏、胸腺、前列腺、睾丸、小肠、结肠、骨骼肌、脑、胎盘、肺、肾脏、卵巢、胰腺和肝脏中均有表达 (PubMed:26856698, PubMed:7488019) 。LAMP-2C 亚型在小肠、结肠、心脏、脑和骨骼肌中均有表达，在肾脏和胎盘中表达较低 (PubMed:26856698) 。诱导：在外周血 B 细胞中，LAMP-2A、LAMP-2B 和 LAMP-2C 同工型在通过 Toll 样受体 TLR7 或 TLR9 刺激免疫反应的治疗后表达上调。异构体 & 翻译后修饰： P13473 有 3 种异构体：P13473-1：44961 Da (预测值)；P13473-2：44956 Da (预测值)；P13473-3：45170 Da (预测值)。 O-和 N-糖基化；16 个 N-连接的聚糖中有一些是聚乳糖胺聚糖。亚基：单体 (PubMed:18644871，PubMed:25342746)。同源二聚体 (PubMed:25342746)。同源三聚体 (PubMed:25342746)。形成大型同源寡聚体 (PubMed:18644871)。通过其胞质区与 HSPA8 相互作用；HSPA8 介导具有 KFERQ 基序的蛋白质募集至溶酶体表面，以进行分子伴侣介导的自噬 (PubMed:25342746，PubMed:36586411)。与溶酶体腔内的 HSP90 相互作用；这增强了 LAMP2 的稳定性 (基于相似性)。与 MLLT11 相互作用 (PubMed:24880125)。与 ABCB9 相互作用 (PubMed:22641697)。与 FURIN 相互作用 (PubMed:32295904)。与 CT55 相互作用；这种相互作用可能对 LAMP2 蛋白的稳定性至关重要 (PubMed:36481789)。与 TMEM175 相互作用；抑制 TMEM175 的质子通道活性 (PubMed:37390818)。与 RAB7A 和 RUFY4 形成三元复合物 (通过 RUN 结构域)；与 RAB7A 的相互作用由 RUFY4 介导 (通过 RUN 和卷曲螺旋结构域)(基于相似性)。