Beta Actin (Fruit Fly) 抗体 (HY-P81254)

ACT1_DROME; Dro-actin; Actin 5C; Act5C; Fruit Fly Actin; β-actin; β actin;

反应种属:Drosophila
抗原名称:Beta Actin (Fruit Fly)
应用:WB
标记:Unconjugated

用户验证的应用

经验证应用

项目详情
WBWestern blot analysis of extracts from Fruit Fly (lane 2, 20ug) using Beta Actin (Fruit Fly) Antibody ( HY-P81254 ). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

推荐稀释比例

  • WB1:1000-5000

详细信息

免疫原Recombinant Fruit Fly Actin.
宿主/亚型Rabbit / IgG
抗体类型Polyclonal
全称Beta Actin (Fruit Fly) 抗体
理论分子量42 kDa
实际/观测分子量42 kDa 请注意:因蛋白存在修饰或聚体等情况,以实测为准,预测仅为参考。
产品形式溶液
偶联物Non-conjugated
储存缓冲液Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
储存条件保存条件 & 期限: Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles. 运输条件: Shipping with blue ice.
纯化方式Affinity Purified
Gene ID31521 [ NCBI ]
RRIDAB_3103122
UniProt IDP10987

背景信息

功能: β-肌动蛋白:肌动蛋白是高度保守的蛋白质,参与多种类型的细胞运动,在所有真核细胞中普遍表达;存在多种异构体,参与细胞骨架结构、细胞运动、染色体移动和肌肉收缩等多种细胞功能。 亚细胞定位: 细胞质、细胞骨架 亚基: 与 Rab6 相互作用