6X His Tag (C-terminal) 抗体 (HY-P80959)

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抗原名称：His-Tag

应用：WB, IP

标记：Unconjugated

用户验证的应用详细信息背景信息

用户验证的应用

经验证应用

项目	详情
WB	Western blot analysis of extracts from HY-P70499 (lane 2(50 ng), HY-P72468 (lane 3(50 ng), HY-P7993A (lane 4(50 ng) and HY-P70620 (lane 5(50 ng) using 6X His-tag (C-terminal) ( HY-P80959 ) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

项目

详情

Western blot analysis of extracts from HY-P70499 (lane 2(50 ng), HY-P72468 (lane 3(50 ng), HY-P7993A (lane 4(50 ng) and HY-P70620 (lane 5(50 ng) using 6X His-tag (C-terminal) ( HY-P80959 ) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

详细信息

免疫原Synthetic peptide corresponding to His-tag.

宿主/亚型Rabbit / IgG

抗体类型Polyclonal

全称6X His Tag (C-terminal) 抗体

理论分子量30 kDa

实际/观测分子量32 kDa 请注意：因蛋白存在修饰或聚体等情况，以实测为准，预测仅为参考。

产品形式液体

偶联物Non-conjugated

储存缓冲液Supplied in 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

储存条件保存条件 & 期限: Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles. 运输条件: Shipping with blue ice.

RRIDAB_3102774

背景信息

功能： 6X His 标签是一种由六个组氨酸残基组成的常用亲和标签。六聚组氨酸 (HEXA-HIS) 可结合含有过渡金属离子 (如镍 (Ni 2+ ) 或钴 (Co 2+ )) 的亲和层析介质，因此可用于蛋白质纯化。