用户验证的应用
经验证应用
| 项目 | 详情 |
|---|---|
| WB | Western blot analysis of c-Myc was performed by loading 0.5 μg, 1 μg and 2 μg recombinant Myc-tagged Intelectin-1/ITLN1 protein onto a 4-20% GelHP Protein Gel (Tris-Gly). Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot ® Turbo ™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST ( HY-K1025 ) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) ( HY-P80954A ) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit ( HY-K1005 ). |
推荐稀释比例
- WB1:1000-1:10000
- IP1:200
详细信息
免疫原Synthetic peptide EQKLISEEDL coupled to KLH.
宿主/亚型Rabbit / IgG
抗体类型Polyclonal
全称c-Myc Tag 抗体
产品形式液体
偶联物Non-conjugated
储存缓冲液Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
储存条件保存条件 & 期限: Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
运输条件: Shipping with blue ice.
纯化方式Protein A affinity purified.
背景信息
功能： c-Myc 标签：Myc 标签是来源于 c-Myc 蛋白的短肽标签，本身无生物学功能，但可用于靶蛋白的检测、定位、纯化以及相互作用研究。