Phospho-JNK1 (Thr183/Tyr185) 抗体 (HY-P88056)

AI849689 antibody; c Jun N terminal kinase 1 antibody; C-JUN kinase 1 antibody; c-Jun N-terminal kinase 1 antibody; EC 2.7.11.24 antibody; JNK 1 antibody; JNK antibody; JNK-46 antibody; JNK1A2 antibody; JNK21B1/2 antibody; MAP kinase 8 antibody; MAPK 8 antibody; mapk8 antibody; Mitogen activated protein kinase 8 antibody; MK08_HUMAN antibody; p54 gamma antibody; Prkm8 antibody; Protein kinase JNK1 antibody; Protein kinase, mitogen-activated, 8 antibody; SAPK 1 antibody; SAPK gamma antibody; SAPK

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反应种属：Human, Mouse, Rat

抗原名称：Phospho-JNK1 (Thr183/Tyr185)

应用：WB, FCM, IF/ICC, IHC-F, IHC-P

标记：Unconjugated

用户验证的应用详细信息背景信息

用户验证的应用

经验证应用

项目	详情
IHC-P	Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue using Phospho-JNK1 (Thr183/Tyr185) Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P88056,1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using Phospho-JNK1 (Thr183/Tyr185) Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P88056,1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam. Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using Phospho-JNK1 (Thr183/Tyr185) Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P88056,1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam. Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue using Phospho-JNK1 (Thr183/Tyr185) Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P88056,1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Phospho-JNK1 (Thr183/Tyr185) Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P88056,1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam. Immunohistochemical analysis of paraffin-embedded human lymphoma tissue using Phospho-JNK1 (Thr183/Tyr185) Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P88056,1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

项目

详情

IHC-P

Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue using Phospho-JNK1 (Thr183/Tyr185) Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P88056,1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using Phospho-JNK1 (Thr183/Tyr185) Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P88056,1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam. Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using Phospho-JNK1 (Thr183/Tyr185) Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P88056,1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam. Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue using Phospho-JNK1 (Thr183/Tyr185) Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P88056,1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Phospho-JNK1 (Thr183/Tyr185) Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P88056,1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam. Immunohistochemical analysis of paraffin-embedded human lymphoma tissue using Phospho-JNK1 (Thr183/Tyr185) Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P88056,1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

详细信息

免疫原KLH conjugated Synthesised phosphopeptide derived from human JNK1 around the phosphorylation site of Thr183/Tyr185: MM(p-T)P(p-Y)VV

宿主/亚型Rabbit / IgG

抗体类型Polyclonal

全称Phospho-JNK1 (Thr183/Tyr185) 抗体

理论分子量48 kDa

实际/观测分子量46/54 kDa 请注意：因蛋白存在修饰或聚体等情况，以实测为准，预测仅为参考。

产品形式溶液

偶联物Non-conjugated

储存缓冲液Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

储存条件保存条件 & 期限: Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles. 运输条件: Shipping with blue ice.

纯化方式affinity purified

Gene ID5599 [ NCBI ]

UniProt IDP45983

背景信息

功能： c-Jun N 端激酶 1 (JNK1) 是一种丝裂原活化蛋白激酶 (MAPK)，调控自噬、凋亡及多种组织的细胞增殖 [1] [2] 。机理上，JNK1 整合细胞因子、活性氧 (ROS) 及代谢信号的应激反应，通过下游转录因子和细胞质底物影响细胞存活和分化 [3] [4] 。在少突胶质祖细胞中，JNK1 控制分支结构、增殖及髓鞘形成，显示其在中枢神经系统发育中的关键作用 [5] 。与 JNK2 和 JNK3 相比，JNK1 独特介导炎症性关节炎反应，并在高脂饮食下促成肝脏脂肪变性 [6] [7] 。在疾病模型中，抑制 JNK1 可减少缺氧诱导自噬并增强癌细胞对化疗药物的敏感性，显示了其同种型特异性治疗潜力 [8] 。由于各 JNK 同种型 ATP 结合位点高度保守，开发 JNK1 特异性抑制剂仍具挑战，但已有计算方法利用分子描述符预测癌症自噬调控的候选抑制剂 [1] 。总体而言，JNK1 是应激信号的核心调控者，在代谢、炎症及神经疾病模型中具有独特作用，为同种型特异性干预的实验设计提供依据 [1] [2] [6] [7] [8] 。亚细胞定位：核表达水平：组织特异性：该基因在慢性粒细胞白血病、Jurkat T 细胞白血病和畸胎瘤细胞系中表达，但在所检测的其他细胞系或正常组织中均未表达。亚基：与 POU5F1 交互