Rabbit IgG Isotype Control (HY-P80879)

Rabbit IgG

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抗原名称：Rabbit IgG

应用：WB, ELISA, FCM, IF/ICC, IF/ICC-Tissue, IHC-F, IHC-P

标记：Unconjugated

用户验证的应用详细信息背景信息

用户验证的应用

经验证应用

项目	详情
WB	Western blot analysis of extracts from Hela (lane 2(20μg), HEK293 (lane 3(20μg) and RAW264.7(lane 4(20μg) using Rabbit IgG Isotype Control mAb ( HY-P80879 ). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

项目

详情

Western blot analysis of extracts from Hela (lane 2(20μg), HEK293 (lane 3(20μg) and RAW264.7(lane 4(20μg) using Rabbit IgG Isotype Control mAb ( HY-P80879 ). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

详细信息

免疫原Synthetic peptide corresponding to Rabbit IgG.

宿主/亚型Rabbit / IgG

抗体类型Polyclonal

全称Rabbit IgG Isotype Control

理论分子量50 kDa

实际/观测分子量50 kDa 请注意：因蛋白存在修饰或聚体等情况，以实测为准，预测仅为参考。

产品形式溶液

偶联物Non-conjugated

储存缓冲液1.Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. 2.Supplied in 0.01M PBS, pH 7.4. Please refer to the lot-specific COA for specific buffer information.

储存条件保存条件 & 期限: Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles. 运输条件: Room temperature in continental US; may vary elsewhere.

纯化方式affinity purified

RRIDAB_3102988

背景信息

功能：同种型对照抗体用于评估由于 Fc 受体结合或其他蛋白质-蛋白质相互作用而导致的靶点抗体的非特异性结合。同型对照抗体应具有与靶点抗体相同的免疫球蛋白类型，并使用相同的浓度。