Anti-GFP antibody [EPR14104] (Ab183734)

特异性

On the basis of low sequence homology, ab183734 is predicted to show no or limited cross-reactivity to RFP and BFP.For the best results in IHC, please use ab183734 on Formalin/PFA-fixed paraffin-embedded sections with heat mediated antigen retrieval.

Anti-GFP antibody [EPR14104] (ab183734) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P and WB. Abcam's high quality manufacturing and validation processes ensure Anti-GFP antibody [EPR14104] (ab183734) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. Anti-GFP antibody [EPR14104] (ab183734) has 24 independent reviews from customers. GFP antibodies are often used as loading controls in Western Blot. Anti-GFP antibody [EPR14104] has been verified in Western Blot samples and detects a band at 27kDa Molecular weight. GFP antibodies are used to visualize proteins labelled with this tag in a variety of applications (for example imaging and Flow cytometry). To enable specific detection of your tagged protein, Anti-GFP antibody [EPR14104] (ab183734) has been validated in Flow Cyt (Intra), ICC/IF, IHC-P and WB. Anti-GFP antibody [EPR14104] (ab183734) specifically detects GFP (UniProt ID: P42212; Molecular weight: 27kDa) and is sold in 100 µL and 1 mL selling sizes. Conjugation-ready, carrier free format available for antibody clone EPR14104 - ab220802. Antibody clone EPR14104 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor ® 488, APC, PE ( ab225314, ab283597, ab303588 ). Green Fluorescent Protein (GFP), originally derived from the jellyfish Aequorea victoria, emits a bright green fluorescence under ultraviolet or blue light. This unique property makes GFP an invaluable tool in molecular and cell biology research. Widely used to tag proteins, GFP allows scientists to visualize and track protein expression, localization and interactions within living cells. Key applications of GFP include fluorescence microscopy (ICC, IHC, IF), flow cytometry (FC) and western blotting (WB). Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents. What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies.