Anti-c-Myc (phospho S62) antibody [EPR17924] (Ab185656)

BHLHE39, MYC, Myc proto-oncogene protein, Class E basic helix-loop-helix protein 39, Proto-oncogene c-Myc, Transcription factor p64, bHLHe39

反应种属:Human, Mouse, Rat
抗原名称:MYC pS62
应用:WB, IHC, FCM, IF/ICC, IP, CUT&RUN-seq, Dot

用户验证的应用

推荐稀释比例

  • IHC-P1/500 Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • WB1/2000
  • ICC/IF1/1000
  • Flow Cyt (Intra)1/150 ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • IP1/50
  • Dot1/1000
  • ChIC/CUT&RUN-seq5 µg

详细信息

免疫原The exact immunogen used to generate this antibody is proprietary information.
宿主/亚型Rabbit / IgG
抗体类型Monoclonal
全称Anti-c-Myc (phospho S62) antibody [EPR17924]
产品形式Liquid
储存缓冲液pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
储存条件运输条件: Conditional Ambient 推荐的短期储存时间: 1-2 weeks 推荐的短期储存条件: +4°C 推荐的长期储存条件: -20°C 储存信息: Avoid freeze / thaw cycle
纯化方式Affinity purification Protein A

背景信息

What is this antibody validated in? Anti-c-Myc (phospho S62) antibody [EPR17924] (ab185656) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), Dot Blot in Human, Mouse, Rat samples. What is the molecular weight of c-Myc? Anti-c-Myc (phospho S62) [EPR17924] (ab185656) specifically detects a band for c-Myc (UniProt: P01106) at a molecular weight of 48kDa. Trusted by the scientific community Anti-c-Myc (phospho S62) [EPR17924] (ab185656) was first used in a scientific publication in 2015 and has been cited over 40 times in peer-reviewed journals. Other related products We have a range of other formats of antibody clone [EPR17924] also available for your convenience: ab185656, Carrier free - ab232691, PE - ab305731, APC - ab305732, HRP - ab305733, Alkaline Phosphatase - ab308652, Alexa Fluor ® 488 - ab309626, Alexa Fluor ® 647 - ab309992, Alexa Fluor ® 594 - ab310355, Alexa Fluor ® 555 - ab311877, Alexa Fluor ® 568 - ab312346, Alexa Fluor ® 750 - ab321295 Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 20µl. Discover our selection of trial-size antibodies. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents. What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies.

文献引用 (64)

PVT1-104aa derived from the 8q24 gene desert promotes colorectal cancer tumorigenesis.

PVT1-104aa 源自 8q24 基因沙漠,可促进结直肠癌的肿瘤发生。

Ma Maoguang, Wang Mingdian, Yang Yufei, Luo Dakui, Dai Weixing, Li Yiwei, Cai Sanjun, Mo Shaobo, Li Qingguo, Li Xinxiang

The protein phosphatase 2A-B56α complex regulates N-Myc degradation in neuroblastoma.

蛋白磷酸酶 2A-B56α 复合物调节神经母细胞瘤中 N-Myc 的降解。

Tran Brian D, Peris Irene, Wurman Ethan, Huang Averie, Hodges Onishi Gabrielle, Hu Jiang, Avelar Rita A, Newman Erika A, DiFeo Analisa, O'Connor Caitlin M, Narla Goutham

The long non-coding RNA UGDH-AS1 encodes an NK-cell-inhibiting micropeptide in triple-negative breast cancers.

长链非编码 RNA UGDH-AS1 编码三阴性乳腺癌中的 NK 细胞抑制微肽。

Zhang Zheng, Li Fanrong, Dai Xiaoxiao, Deng Jieqiong, Guo Binbin, Wang Yirong, Liu Wei, Pan Yacheng, Zhao Tong, Wang Shuang, Li Wanqiu, Jin Congnan, Zhang Hebin, Zhang Shenghua, Zhou Yifeng

G-protein-coupled receptor ADGRG1 drives a protective microglial state in Alzheimer's disease through MYC activation

G蛋白偶联受体ADGRG1通过激活MYC驱动阿尔茨海默病中小胶质细胞的保护性状态。

Beika Zhu ,Andi Wangzhou ,Diankun Yu ,Tao Li ,Rachael Schmidt ,Stacy L De Florencio ,Lauren Chao ,Alicia L Thurber ,Minqi Zhou ,Zeina Msheik ,Yonatan Perez ,Lea T Grinberg ,Salvatore Spina ,Richard M Ransohoff ,Arnold R Kriegstein ,William W Seeley ,Tomasz Nowakowski ,Xianhua Piao

Phosphorylation of c-Myc at Ser62 via the protein kinase activity of acetaldehyde dehydrogenase 18A1 promotes tumor growth.

乙醛脱氢酶 18A1 的蛋白激酶活性使 c-Myc 在 Ser62 位点磷酸化,从而促进肿瘤生长。

Wang Di, Wang Xiao-Ya, Li Wen-Jing, Liang Cheng-Hui, Zou Yu-Xin, Xiao Jin-Wei, Kang Qing-Mei, Wang Jun-Han, Mao Ling, Chen Jun-Jie, Zhai Xue-Jia, Zhuang Ji-Tao, Yang Xian-Yan, Jiang Hong-Ying, Shen Tian-Hua, Liu Jie, Wen Qian, Duan Jiang-Jie, Wang Jun, Yu Shi-Cang

Decursin induces FLT3-ITD acute myeloid leukemia cell apoptosis by increasing the expression of the ubiquitin-conjugase UBE2L6.

Decursin 通过增加泛素结合酶 UBE2L6 的表达来诱导 FLT3-ITD 急性髓系白血病细胞凋亡

Zhang Tianxin, Li Yuchen, Liao Wenhao, Mou Yu, Zhan Xue, Hu Qiongying, Zhao Ziyi, Xiong Daqian

SNORA47 affects stemness and chemotherapy sensitivity via EBF3/RPL11/c-Myc axis in luminal A breast cancer.

SNORA47 通过 EBF3/RPL11/c-Myc 轴影响 Luminal A 型乳腺癌的干细胞特性和化疗敏感性

Han Qilin, Zhou Ying, Dong Zixian, Wang Weitao, Wang Menghan, Pang Mengyang, Song Xinyue, Chen Bo, Zheng Ang

CtBP2 Regulates Wnt Signal Through EGR1 to Influence the Proliferation and Apoptosis of DLBCL Cells.

CtBP2 通过 EGR1 调节 Wnt 信号,从而影响 DLBCL 细胞的增殖和凋亡

Dong Jianfang, Li Lihua, Zhang Xuefei, Yin Xijing, Chen Zucong

Pnky Modulates Neural Stem Cell Proliferation and Differentiation Through Activation of Wnt/β-Catenin Signaling Pathway.

Pnky 通过激活 Wnt/β-catenin 信号通路调节神经干细胞增殖和分化

Wu Haidong, Huang Jing, Li Xiaojing, Song Yali, Chen Xuxiang, Guo Yajie

KAT3B acts as an oncogene in PCa by mediating the succinylation of c-Myc and promoting its stability.

KAT3B 在前列腺癌中发挥癌基因的作用,它介导 c-Myc 的琥珀酰化并促进其稳定性。

Nie Wei, Cha Shifang, Fu Quanwei
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