Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (Ab190565)

RNA binding protein fox-1 homolog 3, Fox-1 homolog C, Neuronal nuclei antigen, NeuN antigen, RBFOX3

反应种属:Human, Mouse, Rat
抗原名称:NeuN
应用:IHC, FCM, IF/ICC
标记:AF647

用户验证的应用

推荐稀释比例

  • IHC-P1/50 ab199093 - Rabbit monoclonal IgG (Alexa Fluor ® 647), is suitable for use as an isotype control with this antibody.
  • ICC/IF1/50
  • IHC-Fr1/1000 Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

详细信息

免疫原The exact immunogen used to generate this antibody is proprietary information.
宿主/亚型Rabbit / IgG
抗体类型Monoclonal
全称Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker
产品形式Liquid
偶联物Alexa Fluor® 647
储存缓冲液pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
储存条件运输条件: Blue Ice 推荐的短期储存时间: 1-2 weeks 推荐的短期储存条件: +4°C 推荐的长期储存条件: -20°C 储存信息: Avoid freeze / thaw cycle|Store in the dark
纯化方式Affinity purification Protein A

背景信息

Product Specifications Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ICC/IF, IHC-Fr, IHC-P in mouse, rat and human samples. Flourescent conjugated antibodies are ideal tools for multiplex IHC and flow cytometry experiments. Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565) specifically detects NeuN (UniProt ID: A6NFN3; Molecular weight: 34kDa) and is sold in 100 uL selling sizes. Quality and Validation Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565) has been cited over 32 times in peer reviewed journals and is trusted by the scientific community. Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565) has 32 independent reviews from customers. Related Products Conjugation-ready, carrier free format available for antibody clone (EPR12763) - ab209898. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents. What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies. Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

文献引用 (79)

Brain-engrafted monocyte-derived macrophages from blood and skull-bone marrow exhibit distinct properties.

脑移植的单核细胞衍生巨噬细胞(来源于血液和颅骨骨髓)表现出不同的特性。

Du Siling, Ou Feiya, Drieu Antoine, Xu Eric Z, Cheng Yumeng, Storck Steffen E, Mamuladze Tornike, Cao Jay, Abduljawad Nora, Bhattarai Bishan, Rustenhoven Justin, Mortimer Niall, Brioschi Simone, Nguyen Khai, Rodrigues Patrick Fernandes, Smirnov Igor, Gibson Daniel, Michael White J, Beatty Wandy, DeNardo David, Li Qingyun, Meers Michael, Han Claudia Z, Sun Na, Ginhoux Florent, Cella Marina, Colonna Marco, Kipnis Jonathan

Temporal and cell-type specific SPAK-NKCC1 disruption following severe TBI in the developing gyrencephalic brain.

在发育中的回脑中,严重脑外伤后SPAK-NKCC1的破坏具有时间和细胞类型特异性。

Hochstetler Alexandra, Courtney Ya'el, Oloko Peace, Baskin Benjamin, Ding-Su Andrew, Stinson Tawny, Alber Elyssa, Chung David Y, McGuone Declan, Haynes Robin, Lehtinen Maria K, Costine-Bartell Beth

Sensory neuron PIEZO1 deletion inhibits dynamic light touch sensitivity in uninjured mice, prevents neuropathic light touch hypersensitivity, and drives compensatory changes in dorsal root ganglia.

感觉神经元 PIEZO1 缺失抑制未受伤小鼠的动态轻触敏感性,防止神经性轻触过敏,并驱动背根神经节的代偿性变化。

Ehlers Vanessa L, Sriram Anvitha, Stuart Bradey A R, Mecca Christina M, Stucky Cheryl L

Arhgef7 is essential for granule cell precursor proliferation and migration during cerebellum development.

Arhgef7 在小脑发育过程中对颗粒细胞前体增殖和迁移至关重要。

Jiménez-Amilburu Vanesa, Ducuing Hugo, Balekoglu Nursen, Sukumaran Sneha, Yam Patricia T, Charron Frédéric

P2X7 receptor-mediated cGAS-STING pathway activation underlies chronic stress-induced depressive-like behaviors.

P2X7 受体介导的 cGAS-STING 通路激活是慢性应激诱发抑郁样行为的基础。

He Xi-Meng, Zhang Yi, Chen Cui-Yuan, Tang Yu-Li Han, Li Xuan

3D immunolabelling of human aortic valves by optimized CUBIC tissue clearing and its application for neural mapping in aortic stenosis.

通过优化 CUBIC 组织透明化技术对人主动脉瓣进行 3D 免疫标记,并将其应用于主动脉瓣狭窄的神经映射。

Bergeon Justine, Hupin David, Hu Jianjiang, Le Guyader Sylvie, Franco-Cereceda Anders, Bäck Magnus

Microglia-neuron crosstalk through Hex-GM2-MGL2 maintains brain homeostasis

小胶质细胞与神经元通过Hex-GM2-MGL2途径进行相互作用,维持大脑稳态。

Maximilian Frosch ,Takashi Shimizu ,Emile Wogram ,Lukas Amann ,Lars Gruber ,Ayelén I Groisman ,Maximilian Fliegauf ,Marius Schwabenland ,Chintan Chhatbar ,Sabrina Zechel ,Hendrik Rosewich ,Jutta Gärtner ,Francisco J Quintana ,Joerg M Buescher ,Thomas Blank ,Harald Binder ,Christine Stadelmann ,Johannes J Letzkus ,Carsten Hopf ,Takahiro Masuda # ,Klaus-Peter Knobeloch # ,Marco Prinz #

Glycocalyx dysregulation impairs blood-brain barrier in ageing and disease

糖萼功能紊乱会损害衰老和疾病状态下的血脑屏障。

Sophia M Shi ,Ryan J Suh ,D Judy Shon ,Francisco J Garcia ,Josephine K Buff ,Micaiah Atkins ,Lulin Li ,Nannan Lu ,Bryan Sun ,Jian Luo ,Ning-Sum To ,Tom H Cheung ,M Windy McNerney ,Myriam Heiman ,Carolyn R Bertozzi ,Tony Wyss-Coray

Meningeal lymphatics-microglia axis regulates synaptic physiology

脑膜淋巴管-小胶质细胞轴调节突触生理

Kyungdeok Kim ,Daviti Abramishvili ,Siling Du ,Zachary Papadopoulos ,Jay Cao ,Jasmin Herz ,Igor Smirnov ,Jean-Leon Thomas ,Marco Colonna ,Jonathan Kipnis

Immunosuppressive Formulations for Immunological Defense against Traumatic Brain Injury

用于免疫防御创伤性脑损伤的免疫抑制制剂

Kelly Lintecum ,Abhirami Thumsi ,Kara Dunn ,Lindsey Druschel ,Sierra Chimene ,David Flores Prieto ,Amberlyn Simmons ,Shivani Mantri ,Arezoo Esrafili ,Srivatsan J Swaminathan ,Mytreyi Trivedi ,Shreya Anandan ,Crystal Willingham ,Alondra Davila ,Sahil Inamdar ,Joslyn L Mangal ,Abhirami P Suresh ,Niveda M Kasthuri ,Madan Mohan Chandra Sekhar Jaggarapu ,Nicole Appel ,Taravat Khodaei ,Nathan D Ng ,Alison Sundem ,Sanmoy Pathak ,George Bjorklund ,Jason Newbern ,Jeffrey Capadona ,Sarah E Stabenfeldt ,Abhinav P Acharya
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