Anti-CD68 antibody [C68/684] (Ab201340)

CD68, Macrosialin, Gp110

反应种属:Human
抗原名称:CD68
应用:WB, IHC, FCM, IF/ICC

用户验证的应用

推荐稀释比例

  • IHC-P1-2 µg/mL Perform heat mediated antigen retrieval with 10 mM Tris with 1 mM EDTA buffer pH 9.0 for 45 minutes at 95°C followed by cooling at RT for 20 minutes. Incubate with primary for 30 minutes at RT.
  • WB1-2 µg/mL
  • ICC/IF1-2 µg/mL
  • Flow Cyt1-2 µg for 10^6 Cells ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

详细信息

免疫原Recombinant Full Length Protein corresponding to Human CD68. P34810
宿主/亚型Mouse / IgG1
抗体类型Monoclonal
全称Anti-CD68 antibody [C68/684]
产品形式Liquid
储存缓冲液pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
储存条件运输条件: Blue Ice 推荐的短期储存时间: 1-2 weeks 推荐的短期储存条件: +4°C 推荐的长期储存条件: -20°C 储存信息: Avoid freeze / thaw cycle
纯化方式Affinity purification Protein A/G

背景信息

Anti-CD68 antibody [C68/684] (ab201340) is a House Mouse Monoclonal antibody and is validated for use in Flow Cyt, ICC/IF, IHC-P, WB in human samples. Anti-CD68 antibody [C68/684] (ab201340) has been cited over 105 times in peer reviewed journals and is trusted by the scientific community. Abcams high quality validation processes ensure Anti-CD68 antibody [C68/684] (ab201340) has high sensitivity and specificity. Anti-CD68 antibody [C68/684] (ab201340) specifically detects CD68 (UniProt ID: P34810; Molecular weight: 35kDa) and is sold in 100 uL selling sizes. Conjugation-ready, carrier free format available for antibody clone C68/684 - ab21398. CD68 is a marker primarily associated with macrophages, playing a crucial role in phagocytosis, antigen presentation, and immune response regulation. CD68 is a biomarker in chronic inflammatory conditions including rheumatoid arthritis and inflammatory bowel disease ab201340 stains macrophages in a wide variety of Human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.

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