Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (Ab205606)

DDDDK epitope tag, DDDK, DYKDDDDK, DYKDDDDK (also known as the FLAG搴 tag sequence), DYKDDDDK epitope tag, DYKDDDDK tag, ECS epitope tag, ECS tag, Enterokinase Cleavage Site epitope tag, Enterokinase Cleavage Site tag, FLAG搴, FLAG搴 tag, ddk, flag, flag tag

抗原名称:DDDDK tag (Binds to FLAG® tag sequence)
应用:WB, IHC, IF/ICC, IP

详细信息

免疫原The exact immunogen used to generate this antibody is proprietary information.
宿主/亚型Rabbit / IgG
抗体类型Monoclonal
产品形式Liquid
纯化方式Affinity purification Protein A
储存缓冲液pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
储存条件运输条件: Conditional Ambient 推荐的短期储存时间: 1-2 weeks 推荐的短期储存条件: +4°C 推荐的长期储存条件: -20°C 储存信息: Avoid freeze / thaw cycle
无载体蛋白No

背景信息

Anti-DDDDK tag (Binds to FLAG ® tag sequence) antibody [EPR20018-251] (ab205606) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ICC/IF, IHC-P, IP and WB. Anti-DDDDK tag (Binds to FLAG ® tag sequence) antibody [EPR20018-251] (ab205606) was first used in a scientific publication in 2019 and has been cited over 115 times in peer reviewed journals. It's performance in Western Blot in human samples is trusted by the scientific community. Abcam's high quality manufacturing and validation processes ensure Anti-DDDDK tag (Binds to FLAG ® tag sequence) antibody [EPR20018-251] (ab205606) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. DDDDK tag (FLAG ® tag) antibodies are used to visualize proteins labelled with this tag in a variety of applications (for example imaging. To enable specific detection of your tagged protein, Anti-DDDDK tag (Binds to FLAG ® tag sequence) antibody [EPR20018-251] (ab205606) has been validated in ICC/IF, IHC-P, IP and WB. Anti-DDDDK tag (Binds to FLAG ® tag sequence) antibody [EPR20018-251] (ab205606) specifically detects DDDDK tag (FLAG ® tag) (UniProt ID: N/A; Molecular weight: 1kDa) and is sold in 100 µL and 1 mL selling sizes. Conjugation-ready, carrier free format available for antibody clone EPR20018-251 - ab236777. Antibody clone EPR20018-251 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor ® 488, Alexa Fluor ® 647, HRP ( ab245892, ab245893, ab245895 ). Top cited recombinant reagent with >243 citations. The DDDDK tag antibody is essential for detecting and purifying DDDDK-tagged fusion proteins, ensuring high specificity and robust detection in various applications Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents. What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies. FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

文献引用 (335)

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由高度迁移的卵巢癌细胞衍生的外泌体 LINC01123 编码的微肽 YG-6 可促进肿瘤进展。

Lei Han, Zhou Zhengwei, Li Chengyuan, Fan Lili, Wu Qihan, Wang Maonan, Guo Ke, Pan Qiong, Mor Gil, Shu Guang, Li Juanni, Yin Gang

Energy-sensing molecule RORγ regulates cholesterol metabolism and immune signaling in diabetic kidney disease and aging.

能量感知分子 RORγ 调节糖尿病肾病和衰老过程中的胆固醇代谢和免疫信号传导。

Liang Zhen, Xiang Jiaqing, Yang Guangyan, Liu Xiaomai, Li Lixing, Li Yanchun, Lu Yan, Kang Lin, Chen Yuanli, Ma Chuanrui, Yang Shu

GCN5 drives MASLD progression through LXRα/SREBP1c signaling pathway-mediated de novo lipogenesis.

GCN5 通过 LXRα/SREBP1c 信号通路介导的从头脂肪生成驱动 MASLD 进展。

Xiao Hai-Tao, Song Peng, Jin Jing, Wang Yong-Dan, Xu Yin-Yue, Wu He, Shao Xue-Xue, Gan Qi-Feng, He Jian, Wen Yan-Rong, Zhu Jing, Chen Bao-Yi, Zhou Ping, Lai Li-Na, Yang Hua, Li Ping, Zheng Zu-Guo

TrxR2 Lactylation Facilitates Mitochondrial Protection and Endothelial Ferroptosis Resistance in Diabetic Cardiomyopathy.

TrxR2 乳酸化促进糖尿病心肌病中的线粒体保护和内皮细胞铁死亡抵抗。

Li Su, Liu Muyin, Chen Chao, Li Xinyan, Yan Xiaopei, Zhu Wentao, Qiu Wenyan, Li Qiyu, Sun Xiangyu, Huang Chao, Yin Ming, Chen Zhangwei, Lu Yao, Ge Junbo, Kong Xiangqing, Qian Juying, Chen Yuqiong

The therapeutic potential and mechanisms of targeting the PTBP1/Nogo-A/NgR axis in PTSD induced by single prolonged stress in mice.

针对PTBP1/Nogo-A/NgR轴治疗小鼠单次长期应激诱发的PTSD的治疗潜力和机制。

Loss of Frmd5 Inhibits Jak2-Stat3 Signalling Pathway and Impairs Cell Apoptosis During Vagina Luminal Formation in Puberty Mice.

Frmd5 的缺失抑制了 Jak2-Stat3 信号通路,并损害了青春期小鼠阴道腔形成过程中的细胞凋亡。

Su Tiantian, Wang Zhenbin, Wu Yunjiao, Li Qianchen, Tian Li, Li Xiaowei, Zhang Jing, Yu Miao, Xing Yuqi, Du Juan, Zhao Lijun, Zhan Jun, Zhang Hongquan

FBXO6 regulates colon cancer migration and invasion via ITGB1 ubiquitination and downstream signaling.

FBXO6 通过 ITGB1 泛素化和下游信号传导调节结肠癌细胞的迁移和侵袭。

Ren Niansheng, Cheng Long, Huang Zijian, Hu Xuchen, Chi Fengxu, Zhu Yuekun, Wang Gang

TRIP13 promotes the expansion and immunosuppression of CD4(+)Foxp3(+) regulatory T cells by sustaining HAT1 stability.

TRIP13 通过维持 HAT1 的稳定性来促进 CD4(+)Foxp3(+) 调节性 T 细胞的扩增和免疫抑制。

He Tianzhen, Zhao Liwen, Feng Chu-Ting, Zhao Li-Ya, Jing Shengnan, Yang Han, Wang Ke, Ye Siyu, Zhao Yingchun, Yu Ying, Fu Zhuting, Chou Chon-Kit, Chen Xin, Gao Yong-Jing

SOX21 suppresses glioblastoma growth by repressing AP-1 activity.

SOX21通过抑制AP-1活性来抑制胶质母细胞瘤的生长。

MYLK4 promotes colorectal cancer progression by regulating lipid metabolism reprogramming via targeting ferroptosis.

MYLK4 通过靶向铁死亡来调节脂质代谢重编程,从而促进结直肠癌的进展。

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