Anti-LAMP1 antibody [H4A3] - Lysosome Marker (Ab25630)

推荐稀释比例

• IHC-P1 µg/mL Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
• WB1/10000
• ICC/IF5-10 µg/mL
• Flow Cyt (Intra)1/20 ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Anti-LAMP1 antibody [H4A3] (ab25630) is a House Mouse Monoclonal antibody and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P, WB in human samples. Anti-LAMP1 antibody [H4A3] (ab25630) has been cited over 285 times in peer reviewed journals and is trusted by the scientific community. Abcams high quality validation processes ensure Anti-LAMP1 antibody [H4A3] (ab25630) has high sensitivity and specificity. Anti-LAMP1 antibody [H4A3] (ab25630) has 23 independent reviews from customers. Anti-LAMP1 antibody [H4A3] (ab25630) specifically detects LAMP1 (UniProt ID: P11279; Molecular weight: 42kDa) and is sold in 100 ug selling sizes. Antibody clone H4A3 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488 ( ab187591 ). Lysosomal-associated membrane protein 1 (LAMP1) is a crucial component of the lysosomal membrane, extensively studied in neuroscience and cell biology. It plays a significant role in lysosomal function, including the degradation of cellular waste and the regulation of autophagy. Alterations in LAMP1 expression are associated with various diseases, such as cancer, neurodegenerative disorders like Alzheimer's disease (AD), and lysosomal storage diseases. Research on LAMP1 helps in understanding the pathology of these conditions and the underlying cellular dysfunction, highlighting its importance in the broader study of disease mechanisms. Although there are publications stating this antibody works with Mouse species (PMID 18840681), and previous batches gave positive staining on murine cells, recent batches are failing to react with this species (from customer feedback). Further feedback using this antibody with Mouse tissues would be very welcome. This product was changed from ascites to tissue culture supernatant on 3rd September 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team. REACH authorisation Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances. It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.