Anti-Cleaved Caspase-3 antibody (AB2302)

Product Specifications Anti-Cleaved Caspase-3 antibody (ab2302) is a rabbit polyclonal antibody and is validated for use in WB in human, recombinant fragment samples. Anti-Cleaved Caspase-3 antibody (ab2302) specifically detects Cleaved Caspase-3 (UniProt ID: P42574; Molecular weight: 17kDa) and is sold in 50 µg selling sizes. Quality and Validation Abcam's high quality validation processes ensure Anti-Cleaved Caspase-3 antibody (ab2302) has high sensitivity and specificity. Anti-Cleaved Caspase-3 antibody (ab2302) has been cited over 1368 times in peer reviewed journals and is trusted by the scientific community. Anti-Cleaved Caspase-3 antibody (ab2302) has 55 independent reviews from customers. Target Information Cleaved Caspase-3 is a crucial marker of apoptosis, the programmed cell death process. It is the activated form of Caspase-3, resulting from proteolytic cleavage. Cleaved Caspase-3 plays a vital role in executing apoptosis by cleaving various cellular substrates, leading to cell dismantling. Researchers use Cleaved Caspase-3 as a reliable indicator of apoptosis in studies of cancer, neurodegenerative diseases, and other conditions involving cell death. Its detection is essential for understanding the mechanisms of apoptosis and developing targeted therapies. In cancer research, cleaved Caspase-3 is used to assess the effectiveness of anti-cancer therapies that induce apoptosis in tumor cells. Elevated levels of cleaved Caspase-3 have been associated with better prognosis in certain cancers, such as oral tongue squamous cell carcinoma. Additionally, cleaved Caspase-3 has been implicated in non-apoptotic roles, such as promoting angiogenesis and chemotherapy resistance. Neurodegenerative Diseases: In neurodegenerative diseases like Alzheimer's (AD) and Parkinson's (PD) disease, cleaved Caspase-3 is involved in the apoptotic pathways that lead to neuronal cell death. For instance, caspase-3-cleaved Tau aggregates more easily than full-length Tau, contributing to the pathology of Alzheimer's disease. Understanding the role of cleaved Caspase-3 in these diseases helps researchers develop targeted therapies to mitigate neuronal loss and disease progression. This product is manufactured by BioVision, an Abcam company and was previously called 3015 Anti-Caspase-3 (Active) Polyclonal Antibody. Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase 3 has been extensively studied and implicated to play an important role in apoptosis. Active caspase 3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP). This affinity purified antibody recognizing the active forms of caspase-3 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.