Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (Ab213224)

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, HIST1H3C, H3FC, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K4me3, H3K4me, H3K4

反应种属:Human, Mouse, Rat
抗原名称:H3C1 tri methyl K4
应用:WB, FCM, IF/ICC, IP, ChIP, CUT&Tag, ChIP-seq, CUT&RUN-seq, Dot, Peptide Array

用户验证的应用

推荐稀释比例

  • WB1/1000 We recommend to use 2% BSA as blocking and antibody dilution buffer.
  • ICC/IF1/500
  • IP1/30
  • ChIP2 µg for 25 µg chromatin
  • ChIC/CUT&RUN-seq2 µg
  • ChIP-seq4 µg for 30 µg chromatin
  • CUT&Tag1/100
  • Dot1/1000
  • PepArr0.1 µg/mL

详细信息

免疫原The exact immunogen used to generate this antibody is proprietary information.
宿主/亚型Rabbit / IgG
抗体类型Monoclonal
全称Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade
产品形式Liquid
储存缓冲液pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
储存条件运输条件: Conditional Ambient 推荐的短期储存时间: 1-2 weeks 推荐的短期储存条件: +4°C 推荐的长期储存条件: -20°C 储存信息: Avoid freeze / thaw cycle
纯化方式Affinity purification Protein A

背景信息

Product Specifications Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224) is a rabbit recombinant monoclonal antibody and is validated for use in CUT&Tag, ChIC/CUT&RUN-seq, ChIP, ChIP-seq, Dot Blot, Flow Cyt (Intra), ICC/IF, IP, PepArr, WB in human, mouse and rat samples. Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224) specifically detects Histone H3 Tri Methyl-K4 (UniProt ID: P68431; Molecular weight: 15kDa) and is sold in 100 µL and 1 mL selling sizes. Quality and Validation Abcam's high quality manufacturing and validation processes ensure Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224) has been cited over 22 times in peer reviewed journals and is trusted by the scientific community. Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224) has 5 independent reviews from customers. Related Products Conjugation-ready, carrier free format available for antibody clone EPR20551-225 - ab313500. Antibody clone EPR20551-225 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647, PE ( ab237340, ab237341, ab237342 ). Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents. What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies.

文献引用 (66)

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Epigenome and interactome profiling uncovers principles of distal regulation in the barley genome.

表观基因组和相互作用组分析揭示了大麦基因组远端调控的原理。

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Isocitrate dehydrogenase 1 primes group-3 medulloblastomas for cuproptosis

异柠檬酸脱氢酶 1 启动 3 组髓母细胞瘤发生铜凋亡

Derek Dang ,Akash Deogharkar ,John McKolay ,Kyle S Smith ,Pooja Panwalkar ,Simon Hoffman ,Wentao Tian ,Sunjong Ji ,Ana P Azambuja ,Siva Kumar Natarajan ,Joanna Lum ,Jill Bayliss ,Katie Manzeck ,Stefan R Sweha ,Erin Hamanishi ,Matthew Pun ,Diya Patel ,Sagar Rau ,Olamide Animasahun ,Abhinav Achreja ,Martin P Ogrodzinski ,Jutta Diessl ,Jennifer Cotter ,Debra Hawes ,Fusheng Yang ,Robert Doherty ,Andrea T Franson ,Allison R Hanaford ,Charles G Eberhart ,Eric H Raabe ,Brent A Orr ,Robert J Wechsler-Reya ,Brandon Chen ,Costas A Lyssiotis ,Yatrik M Shah ,Sophia Y Lunt ,Ruma Banerjee ,Alexander R Judkins ,John R Prensner ,Carl Koschmann ,Sebastian M Waszak ,Deepak Nagrath ,Marcos Simoes-Costa ,Paul A Northcott ,Sriram Venneti

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CREPT 是三阴性乳腺癌转移所必需的,它通过基于染色质环的协同基因调控发挥作用

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Unfolded protein response kinase PERK supports survival and metastasis of circulating tumor cell clusters via SAM synthesis and H3K4me3-dependent PDGFB signaling.

未折叠蛋白反应激酶 PERK 通过 SAM 合成和 H3K4me3 依赖的 PDGFB 信号传导支持循环肿瘤细胞簇的存活和转移。

Tang Rui, Sun Yan, Deng Ao, Liu Jiahe, Dai Peijin, Chen Jing, Deng Chaoqun, Liu Hui, Hai Yuhang, Tong Yanran, Du Yan-E, Liu Manran, Luo Haojun

Co-condensation between transcription factor and cBAF selectively modulates chromatin remodeling and gene expression.

转录因子与 cBAF 的共凝聚选择性地调节染色质重塑和基因表达。

Ye Xifu, Xu Lin, Sun Xuejiao, Gai Yanzhi, Yang Haixia, Sun Qiang, Song Jiayi, Huang Peiqi, Zhou Qianru, Li Yaxu, Li Na, Jin Jiali, Li Yang, Rao Wei, Lu Huasong, Wang Ping, Chang Chunmei, Lu Yi

Symmetric adenine methylation is an essential DNA modification in the early-diverging fungus Rhizopus microsporus.

对称性腺嘌呤甲基化是早期分化的真菌小孢根霉中一种重要的DNA修饰

Lax Carlos, Mondo Stephen J, Martínez José F, Muszewska Anna, Baumgart Leo A, Pérez-Ruiz José A, Carrillo-Marín Pablo, LaButti Kurt, Lipzen Anna, Zhang Yu, Guo Jie, Ng Vivian, Navarro Eusebio, Pawlowska Teresa E, Grigoriev Igor V, Nicolás Francisco E, Garre Victoriano

Reconstructing epigenomic dynamics through a single-cell multi-epigenome data integration framework

通过单细胞多表观基因组数据整合框架重建表观基因组动态

Takeru Fujii,Kosuke Tomimatsu,Michiko Kato,Miho Ito,Shoko Sato,Hitoshi Kurumizaka,Yuko Sato,Kazumitsu Maehara,Hiroshi Kimura,Akihito Harada,Yasuyuki Ohkawa

Oncogenic role of RARG rearrangements in acute myeloid leukemia resembling acute promyelocytic leukemia

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Feng Wang # ,Luyao Zhao # ,Yun Tan # ,Xufeng Cen # ,Huan Gao ,Huimin Jiang ,Ying Liu ,Yunxuan Li ,Tingting Zhang ,Chenxi Zhao ,Ting Shi ,Guilin Xu ,Churan Wang ,Jiong Hu ,Xia Li ,Ya-Zhen Qin ,Kankan Wang ,Hong-Hu Zhu ,Ke Li

Chromatin factor YY1 controls fetal hematopoietic stem cell migration and engraftment in mice

染色质因子YY1控制小鼠胎儿造血干细胞的迁移和植入。

Sahitya Saka ,Zhanping Lu ,Yinghua Wang ,Peng Liu ,Deependra K Singh ,Junki P Lee ,Carmen G Palii ,Tyler R Alvarez ,Anna Lfv Assumpção ,Xiaona You ,Jing Zhang ,Marjorie Brand ,Michael L Atchison ,Xuan Pan
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