Anti-CD68 antibody [EPR20545] (Ab213363)

CD68, Macrosialin, Gp110

反应种属:Human
抗原名称:CD68
应用:WB, IHC, IF/ICC, MIHC

用户验证的应用

推荐稀释比例

  • IHC-P1/8000 Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • mIHC1/500 Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • WB1/1000
  • ICC/IF1/100

详细信息

免疫原The exact immunogen used to generate this antibody is proprietary information.
宿主/亚型Rabbit / IgG
抗体类型Monoclonal
全称Anti-CD68 antibody [EPR20545]
产品形式Liquid
储存缓冲液pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
储存条件运输条件: Conditional Ambient 推荐的短期储存时间: 1-2 weeks 推荐的短期储存条件: +4°C 推荐的长期储存条件: -20°C 储存信息: Avoid freeze / thaw cycle
纯化方式Affinity purification Protein A

背景信息

Product Specifications Anti-CD68 antibody [EPR20545] (ab213363) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ICC/IF, IHC-P, WB, mIHC in human samples. Anti-CD68 antibody [EPR20545] (ab213363) specifically detects CD68 (UniProt ID: P34810; Molecular weight: 35kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL). Quality and Validation Abcam's high quality manufacturing and validation processes ensure Anti-CD68 antibody [EPR20545] (ab213363) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. Anti-CD68 antibody [EPR20545] (ab213363) has been cited over 118 times in peer reviewed journals and is trusted by the scientific community. Anti-CD68 antibody [EPR20545] (ab213363) has 5 independent reviews from customers. Related Products Conjugation-ready, carrier free format available for antibody clone EPR20545 - ab227458. Antibody clone EPR20545 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 647, Alexa Fluor® 488, Alexa Fluor® 594, Alexa Fluor® 555 ( ab224029, ab277276, ab277938, ab280860 ). Target Information CD68 is a transmembrane glycoprotein primarily found on macrophages, essential for phagocytosis and antigen presentation. It plays a role in various pathological conditions, such as neuroinflammatory diseases, by clearing cellular debris and modulating immune responses. CD68 expression is linked to several diseases involving macrophage activity, including cancer, neuroinflammation, granular cell tumor, and histiocytic sarcoma. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents. What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies.

文献引用 (234)

Identification of altered immune landscape at single-cell resolution in NSCLC brain metastasis and its association with poor immune checkpoint inhibitor responses.

在单细胞分辨率下识别非小细胞肺癌脑转移中改变的免疫景观及其与免疫检查点抑制剂反应不良的关系。

Bai Menglin, Yin Tianwen, Li Xiaohui, Wang Peiliang, Lei Tianyu, Jin Peng, Li Feng, Guo Hongbo, Guo Xiaokang, Sun Xiao, Li Yan, Zou Bingwen, Yu Jinming, Liu Chao, Teng Feifei

A proteogenomic atlas of 1032 brain metastases identifies molecular subtypes, immune landscapes, and therapeutic vulnerabilities.

对 1032 例脑转移瘤进行蛋白质基因组图谱分析,确定了分子亚型、免疫图谱和治疗脆弱性。

Yang Zhenyu, Wei Shiyou, Duan Hao, Wang Xiuqi, Zhang Dainan, Kiang Karrie Mei-Yee, Deng Yulan, Yang Yuanzhong, Ku Yin, Hu Wanming, Zou Chaowei, Tang Meiqin, Jiang Yu, Liu Yu, Lan Hongbin, Chen Zexin, Leung Gilberto Ka-Kit, Jia Wang, Mou Yonggao, Liu Lunxu, Zhang Gao

Efferocytosis-Driven Polyamine Metabolism in Macrophages Enhances Cancer Stem Cell Enrichment after Chemotherapy in Ovarian Cancer.

巨噬细胞中胞吞作用驱动的多胺代谢增强了卵巢癌化疗后癌症干细胞的富集。

Li Wenhan, Ying Feiquan, Pang Xinkai, Wu Qiulei, Li Guoqing, Huang Lin, Xin Jiayu, Liu Xiaoli, Liu Pan, Xu Xiaohan, Tan Shuran, Gao Yumei, Liu Tong, Sun Si, Wang Xiaoman, Wen Yiping, Cai Liqiong, Du Shi, Zhang Yuan, Cai Jing

Integrative Multi-Omics Analysis Uncovers Immunological Phenotypes Predictive of Combinatorial Immunotherapy Response in Gastric Cancer.

整合多组学分析揭示了预测胃癌联合免疫疗法反应的免疫表型。

Wang Jianchao, Zhang Wenfang, Zhang Jiyang, Zhao Chenhui, Zhang Wei, Fang Menghan, Chen Fangfang, Wu Zhida, Xu Xiaoya, Yu Ziqing, Zhu Qiong, Shi Yi, Zhang Dadong, Chen Xiaofeng, Chen Gang

A STING signaling relay from tumor cells to macrophages mediates the improved efficacy of combination chemotherapy in pancreatic cancer.

肿瘤细胞向巨噬细胞传递的 STING 信号介导了联合化疗在胰腺癌治疗中疗效的提高。

Ding Honglu, Mao Yize, Yao Zehui, Xing Kaili, Yang Qiuxia, Wang Ruiqi, Wang Jun, Liu Yongxiang, Guo Hui, Wang Zining, Wang Xiaojuan, Wang Jinheng, Xue Jing, Li Shengping, Xia Xiaojun

Single-cell and spatial profiling highlights TB-induced myofibroblasts as drivers of lung pathology.

单细胞和空间分析表明,结核病诱导的肌成纤维细胞是肺部病理的驱动因素。

Mbano Ian M, Liu Nuo, Wadsworth Marc H 2nd, Chambers Mark J, Mpotje Thabo, Asowata Osaretin E, Nyquist Sarah K, Nargan Kievershen, Ramsuran Duran, Karim Farina, Hughes Travis K, Bromley Joshua D, Krause Robert, Naidoo Threnesan, Tezera Liku B, Reichmann Michaela T, Ganchua Sharie Keanne, Kløverpris Henrik N, Dullabh Kaylesh J, Madansein Rajhmun, Triana Sergio, Steyn Adrie J C, Berger Bonnie, Marakalala Mohlopheni J, Pollara Gabriele, Fortune Sarah M, Flynn JoAnne L, Elkington Paul T, Shalek Alex K, Leslie Alasdair

Enhancing gastric cancer immunotherapy: Insights from multi-omics analysis and innovations in photodynamic-chemotherapy nanoplatforms.

增强胃癌免疫疗法:来自多组学分析和光动力化学疗法纳米平台创新的见解。

Metabolic Syndrome Develops Cardia Cancer via Nuclear Factor-E2-related Factor 2-Programmed Death-Ligand 1 Signaling.

代谢综合征通过核因子-E2相关因子2-程序性死亡配体1信号通路发展为心脏病。

Kusano Keisuke, Uno Kaname, Tamahara Toru, Asano Naoki, Sudo Koichiro, Tanabe Mizuki, Ogasawara Koya, Kanno Takeshi, Koike Tomoyuki, Shimizu Ritsuko, Masamune Atsushi

Reprogramming of cholesterol sensing in epithelial cells supports pancreatic inflammation.

上皮细胞中胆固醇感知的重编程促进胰腺炎症。

Milan Giulia, Mareninova Olga A, Fantuz Marco, Spacci Martina, Paoli Carlotta, Pineda Jerik A, Noè Roberta, Calciolari Beatrice, Zoncu Roberto, Gukovskaya Anna S, Carrer Alessandro

Prognostic integration of tumor microenvironment and parthanatos-related genes in gastric cancer: a machine learning-driven risk model and immune landscape profiling.

胃癌中肿瘤微环境和与细胞死亡相关的基因的预后整合:机器学习驱动的风险模型和免疫景观分析。

Liu Lei, Wu Min, Liu Yi
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