KRAS4B is required for placental development.

Beyond its well-established role in cancer, KRAS is also crucial for embryogenesis, as its absence leads to embryonic lethality. However, the precise mechanisms underlying the developmental functions of KRAS, as well as the respective roles of its two splicing isoforms, KRAS4A and KRAS4B, remain incompletely characterized. To address these issues, we generated Kras4A knock-out (Kras4A(-/-)) and Kras4B(-/-) mouse models using CRISPR/Cas9 technology, and compared their phenotypes to those of a Kras(-/-) model, in which both isoforms are simultaneously inactivated. We observed that Kras(-/-) and Kras4B(-/-) embryos show a lethality that starts around E13.5, while Kras4A(-/-) embryos develop normally, with no detectable abnormalities. In contrast, Kras(-/-) embryos displayed a dual phenotype affecting both the heart and placenta, whereas Kras4B(-/-) embryos exhibited only the placental phenotype. The cardiac phenotype was complex, combining ventricular non-compaction, ventricular septal defects, double outlet right ventricle, and overriding aorta, likely resulting from impaired cardiac precursor proliferation. The placental phenotype was characterized by reduced placental size, and a marked decrease in glycogen trophoblast cells, correlating with hypoglycemia and hypoxia in Kras(-/-) and Kras4B(-/-) embryos. Thus, our findings confirm the predominant role of KRAS4B in KRAS-mediated developmental functions, but also suggest hidden functions of KRAS4A. Importantly, this study is the first to identify KRAS as a key regulator of a specific cell differentiation process and to characterize the biological defects caused by its loss.