Abstract
Introduction:
The progression of UC to CACRC involves substantial molecular and cellular alterations. A deeper understanding of these changes is essential for identifying potential therapeutic targets and improving disease outcomes.
Methods:
We performed scRNA-seq on tissue samples from a patient with coexisting UC and CACRC lesions, including normal colon, UC-affected tissue, and CACRC. Cell clustering, differential gene expression, and KEGG pathway enrichment analyses were conducted to characterize cellular heterogeneity and pathway dynamics.
Results:
Thirteen distinct cell clusters were identified, reflecting significant heterogeneity across disease stages. Six major cell types-B cells, T cells, epithelial cells, monocytes, neutrophils, and CMPs-were selected for in-depth analysis. Epithelial cells from UC samples showed marked upregulation of inflammatory genes such as IL-17A, CXCL1, IL-6, MMP3, and TNFAIP3, which were downregulated in CACRC. KEGG analysis revealed IL-17 signaling as a key pathway involved in disease progression. A progressive increase in Tregs, supported by elevated CD25 expression, was observed from normal tissue through UC to CACRC. Furthermore, C-MYC was significantly upregulated in CACRC epithelial cells, suggesting its role in tumor proliferation and metabolic reprogramming.
Conclusion:
This study uncovers dynamic cellular and molecular changes during the transition from UC to CACRC, highlighting IL-17 signaling, Treg expansion, and C-MYC activation as potential drivers of malignancy and targets for future therapeutic intervention.
Keywords:
IL-17 signaling pathway; colorectal cancer; epithelial cells; single-cell RNA sequencing; treg cells; ulcerative colitis.