Analysis of the heavy-chain modification and TSG-6 activity in pathological hyaluronan matrices.

病理性透明质酸基质中重链修饰和TSG-6活性的分析

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作者:Lauer Mark E, Loftis Jacqueline, de la Motte Carol, Hascall Vincent C
During inflammation and developmental processes, heavy chains (HCs) from inter-α-inhibitor (IαI) are covalently transferred to hyaluronan (HA) via the enzyme tumor-necrosis-factor-stimulated-gene 6 (TSG-6) to form a HC-HA complex. In this manuscript, we describe a gel-based assay to detect HC-HA and TSG-6 activity in tissues.

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