Analysis of soybean cultivars response to mosaic and mottle disease caused by soybean yellow mottle mosaic virus.

Soybean yellow mottle mosaic virus is an emerging viral pathogen in leguminous crops including soybean. The present study was carried with 18 soybean cultivars to screen and identify the resistant cultivars to SYMMV infection under controlled environment glasshouse. Agro-inoculation of French bean cv. Arka Sharat with SYMMV produced systemic symptoms of mosaic and chlorotic blotches. Mechanical sap inoculation of 18 soybean cultivars with SYMMV infected French bean leaves produced systemic symptoms like chlorotic spots, chlorotic blotches, mosaic, mottle and veinal mild mottling by 15-20 dpi. The PDI on a scale of 0-5 showed that out of 18 cultivars, only SL-979 cultivar was moderately resistant, seven cultivars were moderately susceptible, and eight cultivars were susceptible, while two cultivars displayed highly susceptible reaction (SL-958 & JS-335). Detection of SYMMV through DAC-ELISA showed the absorbance values of 0.84 to 2.05 at 405 nm. RT-PCR analysis of these cultivars showed amplification of 1065 bp fragment with CP primers. The present study clearly showed that none of the screened soybean cultivar is resistant against SYMMV, suggesting a need for further research into breeding programs that may enhance resistance to SYMMV. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s13337-024-00905-7.