Evaluation of a real-time PCR assay using the LightCycler system for detection of parvovirus B19 DNA

使用 LightCycler 系统对细小病毒 B19 DNA 进行实时 PCR 检测的评估

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作者:Richard S Buller, Gregory Storch

Abstract

We evaluated the artus RealArt Parvovirus B19 LC PCR reagent (artus biotech USA, San Francisco, Calif.) for real-time PCR detection of parvovirus B19 DNA by retesting 71 specimens previously submitted to our laboratory. The artus assay, which produces a quantitative result and provides an internal PCR control, appeared to be slightly more sensitive than our conventional qualitative PCR assay.

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