Microbial diversity in relation to physico-chemical properties of hot water ponds located in the Yamunotri landscape of Garhwal Himalaya.

The current study includes the assessment of physico-chemical characteristics along with the microbial diversity of hot water samples collected from three important sacred hot water springs of the Uttarakhand Himalaya close to the world-famous Hindu shrine Yamunotri temple. Hot water samples were collected for two consecutive years 2015-2016 in two sampling operations each year. A total of fifteen physico-chemical attributes of hot water were recorded. Microbes of hot water ponds were characterized by morphological, biochemical, MALDI-TOF MS, and molecular approaches. A total of twenty-two microbial strains were identified from the Surya Kund with water temperature ranging between 86 °C and 89 °C; twenty-two microbial strains were identified from the Draupadi Kund with water temperature ranging between 65 °C and 69 °C and twenty-one microbial strains were identified from the Yamunotri Tapt Kund with water temperature ranging between 45 °C and 48 °C during the study period. The present study on the assessment of physico-chemical characteristics and thermophilic microbial diversity of all the three hot water springs can be a useful reference for further studies on similar aspects in other parts of the Himalaya. The available data will also help to understand the reasons behind the curative properties of these hot water springs. This baseline information will also be instrumental for the conservation and management of these hot water springs.