Multiplex short tandem repeat profiling of immortalized hepatic stellate cell line Col-GFP HSC

永生化肝星状细胞系 Col-GFP HSC 的多重短串联重复分析

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作者:Steffen K Meurer, David A Brenner, Ralf Weiskirchen

Abstract

Misidentification, cross-contamination and genetic drift of continuous animal cell lines are persistent problems in biomedical research, leading to erroneous results and inconsistent or invalidated studies. The establishment of immortalized hepatic stellate cell line Col-GFP HSC was reported in PLoS One in the year 2013. In the present study a multi loci short tandem repeat signature for this cell line was established that allows for unique cell line authentication.

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