Expression of single-chain Fv fragments in E. coli cytoplasm.

在大肠杆菌细胞质中表达单链Fv片段

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作者:Guglielmi Laurence, Martineau Pierre
The most frequently used approach to produce single-chain Fv fragments (scFv) and Fab in Escherichia coli is to express them in the periplasm of the bacteria. We present here an alternative procedure that uses cytoplasmic expression of soluble active scFv. This can be accomplished by using either specially engineered E. coli strains or hyperstable scFvs.

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