Protocol to isolate dorsal root ganglion neurons from embryonic rats by immunopanning and characterize them using RNAscope and immunofluorescence.

Dorsal root ganglion (DRG) neurons innervate the periphery of the body to transmit somatosensory information, including touch, pain, and temperature. Here, we present a protocol for isolating rat DRG neurons using an immunopanning technique. We describe steps for dissecting DRGs from embryonic day (E)15 rat embryos, triturating ganglionic cells, and purifying DRG neurons. We also detail procedures for mRNA or protein quantification using RNAscope and immunofluorescence. This protocol has potential applications for studying DRG neurons during development and disease. For complete details on the use and execution of this protocol, please refer to Kantarci et al.(1).