Colorimetric and fluorescent probes for real-time naked eye sensing of copper ion in solution and on paper substrate.

In this paper, BT ((E)-2-(4-(4-(bis(pyridin-2-ylmethyl)amino)styryl)-3-cyano-5,5-dimethylfuran-2(5H)-ylidene)malononitrile) with strong donor-π-acceptor structure was synthesized, which showed both colorimetric and fluorescent sensing ability toward Cu(2+) with high selectivity and sensitivity. Job plot and mass spectra measurement revealed a 1 : 1 coordination mode between Cu(2+) and probe BT in ethanol/HEPES (1 : 4 v/v) buffer (pH 7.2) solution, and the binding constant was calculated to be 3.6 × 10(4) M(-1). The colour of BT solution (10 µM) immediately turned from purple red to yellow and the red fluorescence was quenched obviously when a certain amount of Cu(2+) was added, which enabled a dual-channel detection of Cu(2+). A paper strip pre-stained with BT solution was further fabricated and it also showed excellent sensing ability toward Cu(2+) with a detection limit as low as 10(-6) M with the naked eye, which represents better portability and operation simplicity that is favourable for on-site analysis of Cu(2+) in water.